TY - JOUR
T1 - Crystallization and Preliminary Crystallographic Analysis of Recombinant Xenopus laevis Cu, Zn Superoxide Dismutase b
AU - Carugo, K. D.
AU - Collyer, C.
AU - Coda, A.
AU - Carri, M. T.
AU - Battistoni, A.
AU - Bottaro, G.
AU - Polticelli, F.
AU - Desideri, A.
AU - Bolognesi, M.
PY - 1993/8/16
Y1 - 1993/8/16
N2 - The recombinant Cu,Zn superoxide dismutase from the South African frog Xenopus laevis, expressed in E. coli, has been crystallized in a form suitable for high resolution crystallographic investigations. The crystals grow from polyethylene glycol solutions, at pH 6.0, 28° C, and belong to the orthorhombic space group P212121 with unit cell edges a = 73.33, b = 68.86, c = 59.73 Å, one protein dimer (32,000 Mr) per asymmetric unit. Diffraction data have been collected to 3.0 Å resolution, and a molecular replacement solution found for Xenopus laevis superoxide dismutase using the bovine enzyme as search model. The crystallographic R-factor corresponding to this solution is 0.412, in the 15.0-3.0 Å resolution range.
AB - The recombinant Cu,Zn superoxide dismutase from the South African frog Xenopus laevis, expressed in E. coli, has been crystallized in a form suitable for high resolution crystallographic investigations. The crystals grow from polyethylene glycol solutions, at pH 6.0, 28° C, and belong to the orthorhombic space group P212121 with unit cell edges a = 73.33, b = 68.86, c = 59.73 Å, one protein dimer (32,000 Mr) per asymmetric unit. Diffraction data have been collected to 3.0 Å resolution, and a molecular replacement solution found for Xenopus laevis superoxide dismutase using the bovine enzyme as search model. The crystallographic R-factor corresponding to this solution is 0.412, in the 15.0-3.0 Å resolution range.
UR - http://www.scopus.com/inward/record.url?scp=0027279577&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027279577&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1993.1921
DO - 10.1006/bbrc.1993.1921
M3 - Article
C2 - 8352757
AN - SCOPUS:0027279577
VL - 194
SP - 1008
EP - 1011
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -