Crystallization and Preliminary Crystallographic Analysis of Recombinant Xenopus laevis Cu, Zn Superoxide Dismutase b

K. D. Carugo, C. Collyer, A. Coda, M. T. Carri, A. Battistoni, G. Bottaro, F. Polticelli, A. Desideri, M. Bolognesi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The recombinant Cu,Zn superoxide dismutase from the South African frog Xenopus laevis, expressed in E. coli, has been crystallized in a form suitable for high resolution crystallographic investigations. The crystals grow from polyethylene glycol solutions, at pH 6.0, 28° C, and belong to the orthorhombic space group P212121 with unit cell edges a = 73.33, b = 68.86, c = 59.73 Å, one protein dimer (32,000 Mr) per asymmetric unit. Diffraction data have been collected to 3.0 Å resolution, and a molecular replacement solution found for Xenopus laevis superoxide dismutase using the bovine enzyme as search model. The crystallographic R-factor corresponding to this solution is 0.412, in the 15.0-3.0 Å resolution range.

Original languageEnglish
Pages (from-to)1008-1011
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume194
Issue number3
DOIs
Publication statusPublished - Aug 16 1993

Fingerprint

Xenopus laevis
Crystallization
R388
Anura
Dimers
Escherichia coli
Superoxide Dismutase
Diffraction
Crystals
Enzymes
Superoxide Dismutase-1
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Crystallization and Preliminary Crystallographic Analysis of Recombinant Xenopus laevis Cu, Zn Superoxide Dismutase b. / Carugo, K. D.; Collyer, C.; Coda, A.; Carri, M. T.; Battistoni, A.; Bottaro, G.; Polticelli, F.; Desideri, A.; Bolognesi, M.

In: Biochemical and Biophysical Research Communications, Vol. 194, No. 3, 16.08.1993, p. 1008-1011.

Research output: Contribution to journalArticle

Carugo, KD, Collyer, C, Coda, A, Carri, MT, Battistoni, A, Bottaro, G, Polticelli, F, Desideri, A & Bolognesi, M 1993, 'Crystallization and Preliminary Crystallographic Analysis of Recombinant Xenopus laevis Cu, Zn Superoxide Dismutase b', Biochemical and Biophysical Research Communications, vol. 194, no. 3, pp. 1008-1011. https://doi.org/10.1006/bbrc.1993.1921
Carugo, K. D. ; Collyer, C. ; Coda, A. ; Carri, M. T. ; Battistoni, A. ; Bottaro, G. ; Polticelli, F. ; Desideri, A. ; Bolognesi, M. / Crystallization and Preliminary Crystallographic Analysis of Recombinant Xenopus laevis Cu, Zn Superoxide Dismutase b. In: Biochemical and Biophysical Research Communications. 1993 ; Vol. 194, No. 3. pp. 1008-1011.
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