Crystallization and preliminary crystallographic characterization of the extracellular Ig-like domain of human natural killer cell activating receptor NKp44

Claudia Cantoni, Marco Ponassi, Roberto Biassoni, Romana Conte, Andrea Spallarossa, Alessandro Moretta, Lorenzo Moretta, Martino Bolognesi, Domenico Bordo

Research output: Contribution to journalArticle

Abstract

The cytolytic activity of human natural killer (NK) cells is regulated by fine balancing between activating and inhibitory signals related to distinct families of inhibitory and triggering receptor molecules. The recent identification of NKp44, a triggering receptor selectively expressed by activated NK cells and playing an important role in the natural cytotoxicity of these cells, opens the way to the characterization of the mechanisms involved in NKp44-mediated NK-cell activation on a structural basis. For this purpose, the NKp44 extracellular Ig-like domain was cloned, overexpressed, refolded and crystallized. Diffraction intensities were collected to a resolution limit of 2.2 Å on a synchrotron source. NKp44 extracellular Ig-like domain crystals belong to the hexagonal space group P6222 (or P6422), with unit-cell parameters a = b = 60.4, c = 197.2 Å, γ = 120°. Evaluation of the crystal packing parameter suggests the presence of one molecule (13.8 kDa) per asymmetric unit, with a solvent volume of 67%.

Original languageEnglish
Pages (from-to)1843-1845
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume58
Issue number10 I
DOIs
Publication statusPublished - Oct 1 2002

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

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