Crystallization and preliminary X-ray analysis of neural haemoglobin from the nemertean worm Cerebratulus lacteus

A. Pesce, M. Nardini, S. Dewilde, P. Ascenzi, A. F. Riggs, K. Yamauchi, E. Geuens, L. Moens, M. Bolognesi

Research output: Contribution to journalArticle

Abstract

The nemertean worm Cerebratulus lacteus neural tissue haemoglobin (109 amino acids, the shortest known haemoglobin) has been overexpressed in Escherichia coli, purified and crystallized. A highly redundant native data set has been collected at the Cu Kα wavelength to 2.05 Å resolution. The crystals belong to the orthorhombic P212121 space group, with unit-cell parameters a = 42.5, b = 43.1, c = 60.2 Å and one molecule per asymmetric unit. The anomalous difference Patterson map clearly reveals the position of the haem Fe atom, thus paving the way for MAD/SAD structure determination.

Original languageEnglish
Pages (from-to)1897-1899
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume57
Issue number12
DOIs
Publication statusPublished - 2001

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

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    Pesce, A., Nardini, M., Dewilde, S., Ascenzi, P., Riggs, A. F., Yamauchi, K., Geuens, E., Moens, L., & Bolognesi, M. (2001). Crystallization and preliminary X-ray analysis of neural haemoglobin from the nemertean worm Cerebratulus lacteus. Acta Crystallographica Section D: Biological Crystallography, 57(12), 1897-1899. https://doi.org/10.1107/S0907444901015815