Crystallization and preliminary X-ray characterization of the acylphosphatase-like domain from the Escherichia coli hydrogenase maturation factor HypF

Camillo Rosano, Simone Zuccotti, Massimo Stefani, Monica Bucciantini, Giampietro Ramponi, Martino Bolognesi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Maturation of prokaryotic hydrogenase involves several protein factors, among which is the accessory protein HypF, which hosts the consensus sequence of acylphosphatases and a sequence motif common to proteins catalyzing O-carbamoylations. The specific functions of HypF are largely unknown, although it has been observed that CN- and CO ligands at the hydrogenase Ni,Fe active centre originate from carbamoylphosphate. The HypF N-terminal domain (91 residues, acylphosphatase-like domain) has been crystallized in two different crystal forms belonging to the orthorhombic P212121 space group (unit-cell parameters a = 35.5, b = 59.8, c = 87.6 Å) and to the rhombohedral space group R32 (unit-cell parameters a = b = 58.1, c = 155.6 Å in the hexagonal setting).

Original languageEnglish
Pages (from-to)524-525
Number of pages2
JournalActa Crystallographica Section D: Biological Crystallography
Volume58
Issue number3
DOIs
Publication statusPublished - 2002

Fingerprint

Hydrogenase
Escherichia
Crystallization
Escherichia coli
X-Rays
crystallization
proteins
X rays
Proteins
x rays
accessories
Consensus Sequence
Accessories
Carbon Monoxide
cells
Ligands
Crystals
ligands
crystals
acylphosphatase

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Clinical Biochemistry
  • Structural Biology
  • Condensed Matter Physics

Cite this

Crystallization and preliminary X-ray characterization of the acylphosphatase-like domain from the Escherichia coli hydrogenase maturation factor HypF. / Rosano, Camillo; Zuccotti, Simone; Stefani, Massimo; Bucciantini, Monica; Ramponi, Giampietro; Bolognesi, Martino.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 58, No. 3, 2002, p. 524-525.

Research output: Contribution to journalArticle

Rosano, Camillo ; Zuccotti, Simone ; Stefani, Massimo ; Bucciantini, Monica ; Ramponi, Giampietro ; Bolognesi, Martino. / Crystallization and preliminary X-ray characterization of the acylphosphatase-like domain from the Escherichia coli hydrogenase maturation factor HypF. In: Acta Crystallographica Section D: Biological Crystallography. 2002 ; Vol. 58, No. 3. pp. 524-525.
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AU - Ramponi, Giampietro

AU - Bolognesi, Martino

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