Crystallization and preliminary X-ray crystallographic studies on the herpes simplex virus 1 single-stranded DNA binding protein

Marina Mapelli, Paul A. Tucker

Research output: Contribution to journalArticle

Abstract

Crystals of a 60-amino-acid C-terminal deletion mutant of the herpes simplex virus 1 single-stranded DNA binding protein, ICP8, have been grown by hanging drop vapor diffusion. The colorless crystals grow as thin plates to a maximum size of approximately 0.3 mm x 0.3 mm x 0.05 mm. The space group is P212121 with unit cell constants a 101.2 Å, b = 145.8 Å, and c = 162.9 Å. There are one or two molecules of ICP8 per asymmetric unit.

Original languageEnglish
Pages (from-to)219-222
Number of pages4
JournalJournal of Structural Biology
Volume128
Issue number2
DOIs
Publication statusPublished - Dec 1999

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Keywords

  • Crystallization
  • Herpes simplex virus 1
  • ICP8
  • Single-stranded DNA binding protein

ASJC Scopus subject areas

  • Structural Biology

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