CSA and CSB play a role in the response to DNA breaks

Barbara Pascucci, Alessandra Fragale, Veronica Marabitti, Giuseppe Leuzzi, Angelo Salvatore Calcagnile, Eleonora Parlanti, Annapaola Franchitto, Eugenia Dogliotti, Mariarosaria D'Errico

Research output: Contribution to journalArticle

Abstract

CS proteins have been involved in the repair of a wide variety of DNA lesions. Here, we analyse the role of CS proteins in DNA break repair by studying histone H2AX phosphorylation in different cell cycle phases and DNA break repair by comet assay in CS-A and CS-B primary and transformed cells. Following methyl methane sulphate treatment a significant accumulation of unrepaired single strand breaks was detected in CS cells as compared to normal cells, leading to accumulation of double strand breaks in S and G2 phases. A delay in DSBs repair and accumulation in S and G2 phases were also observed following IR exposure. These data confirm the role of CSB in the suppression of NHEJ in S and G2 phase cells and extend this function to CSA. However, the repair kinetics of double strand breaks showed unique features for CS-A and CS-B cells suggesting that these proteins may act at different times along DNA break repair. The involvement of CS proteins in the repair of DNA breaks may play an important role in the clinical features of CS patients.

Original languageEnglish
Pages (from-to)11581-11591
Number of pages11
JournalOncotarget
Volume9
Issue number14
DOIs
Publication statusPublished - Feb 20 2018

Fingerprint

DNA Breaks
G2 Phase
S Phase
DNA Repair
Proteins
Comet Assay
Methane
Histones
Cell Cycle
B-Lymphocytes
Phosphorylation
DNA
Therapeutics

Keywords

  • Cockayne syndrome
  • DNA damage
  • DNA repair
  • γ-H2AX

ASJC Scopus subject areas

  • Oncology

Cite this

CSA and CSB play a role in the response to DNA breaks. / Pascucci, Barbara; Fragale, Alessandra; Marabitti, Veronica; Leuzzi, Giuseppe; Calcagnile, Angelo Salvatore; Parlanti, Eleonora; Franchitto, Annapaola; Dogliotti, Eugenia; D'Errico, Mariarosaria.

In: Oncotarget, Vol. 9, No. 14, 20.02.2018, p. 11581-11591.

Research output: Contribution to journalArticle

Pascucci B, Fragale A, Marabitti V, Leuzzi G, Calcagnile AS, Parlanti E et al. CSA and CSB play a role in the response to DNA breaks. Oncotarget. 2018 Feb 20;9(14):11581-11591. https://doi.org/10.18632/oncotarget.24342
Pascucci, Barbara ; Fragale, Alessandra ; Marabitti, Veronica ; Leuzzi, Giuseppe ; Calcagnile, Angelo Salvatore ; Parlanti, Eleonora ; Franchitto, Annapaola ; Dogliotti, Eugenia ; D'Errico, Mariarosaria. / CSA and CSB play a role in the response to DNA breaks. In: Oncotarget. 2018 ; Vol. 9, No. 14. pp. 11581-11591.
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