C3-fullero-tris-methanodicarboxylic acid protects cerebellar granule cells from apoptosis

M. Bisaglia, B. Natalini, R. Pellicciari, E. Straface, W. Malorni, D. Monti, C. Franceschi, G. Schettini

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Buckminsterfullerenols were recently investigated for their protective properties in different models of acute and chronic neurodegeneration. We tested C3-fullero-tris-methanodicarboxylic acid in our in vitro model of apoptotic neuronal death, which consists of shifting the culture K+ concentration from 25 to 5 mM for rat cerebellar granule cells. The impairment of mitochondrial respiratory function as well as chromatin derangement and fragmentation of DNA in apoptotic oligonucleosomes that occur in these conditions were protected by this compound in a concentration- dependent way. To assess whether antioxidant activity could account for the rescue of cerebellar granule cells from apoptosis, we tested the fullerene derivative under FeSO4-induced oxidative stress and found significant protection. Thus, we visualized membrane and cytoplasmic peroxides and reactive oxygen species and found a significant reduction of the species after 24 h in 5 mM K+ with the fullerene derivative. Such evidence suggests that this compound exerts a protective role in cerebellar granule cell apoptosis, likely reducing the oxidative stress.

Original languageEnglish
Pages (from-to)1197-1204
Number of pages8
JournalJournal of Neurochemistry
Volume74
Issue number3
Publication statusPublished - 2000

Fingerprint

Fullerenes
Oxidative stress
Apoptosis
Derivatives
Peroxides
Oxidative Stress
Chromatin
Rats
Reactive Oxygen Species
Antioxidants
DNA Fragmentation
Membranes
DNA
Cell Membrane
C(3)-fullero-tris-methanodicarboxylic acid
buckminsterfullerenol

Keywords

  • Apoptosis
  • Cerebellar granule cells
  • Fullerene derivative
  • Reactive oxygen species

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Bisaglia, M., Natalini, B., Pellicciari, R., Straface, E., Malorni, W., Monti, D., ... Schettini, G. (2000). C3-fullero-tris-methanodicarboxylic acid protects cerebellar granule cells from apoptosis. Journal of Neurochemistry, 74(3), 1197-1204.

C3-fullero-tris-methanodicarboxylic acid protects cerebellar granule cells from apoptosis. / Bisaglia, M.; Natalini, B.; Pellicciari, R.; Straface, E.; Malorni, W.; Monti, D.; Franceschi, C.; Schettini, G.

In: Journal of Neurochemistry, Vol. 74, No. 3, 2000, p. 1197-1204.

Research output: Contribution to journalArticle

Bisaglia, M, Natalini, B, Pellicciari, R, Straface, E, Malorni, W, Monti, D, Franceschi, C & Schettini, G 2000, 'C3-fullero-tris-methanodicarboxylic acid protects cerebellar granule cells from apoptosis', Journal of Neurochemistry, vol. 74, no. 3, pp. 1197-1204.
Bisaglia M, Natalini B, Pellicciari R, Straface E, Malorni W, Monti D et al. C3-fullero-tris-methanodicarboxylic acid protects cerebellar granule cells from apoptosis. Journal of Neurochemistry. 2000;74(3):1197-1204.
Bisaglia, M. ; Natalini, B. ; Pellicciari, R. ; Straface, E. ; Malorni, W. ; Monti, D. ; Franceschi, C. ; Schettini, G. / C3-fullero-tris-methanodicarboxylic acid protects cerebellar granule cells from apoptosis. In: Journal of Neurochemistry. 2000 ; Vol. 74, No. 3. pp. 1197-1204.
@article{cda0323ec2a64cb9969af07296aca314,
title = "C3-fullero-tris-methanodicarboxylic acid protects cerebellar granule cells from apoptosis",
abstract = "Buckminsterfullerenols were recently investigated for their protective properties in different models of acute and chronic neurodegeneration. We tested C3-fullero-tris-methanodicarboxylic acid in our in vitro model of apoptotic neuronal death, which consists of shifting the culture K+ concentration from 25 to 5 mM for rat cerebellar granule cells. The impairment of mitochondrial respiratory function as well as chromatin derangement and fragmentation of DNA in apoptotic oligonucleosomes that occur in these conditions were protected by this compound in a concentration- dependent way. To assess whether antioxidant activity could account for the rescue of cerebellar granule cells from apoptosis, we tested the fullerene derivative under FeSO4-induced oxidative stress and found significant protection. Thus, we visualized membrane and cytoplasmic peroxides and reactive oxygen species and found a significant reduction of the species after 24 h in 5 mM K+ with the fullerene derivative. Such evidence suggests that this compound exerts a protective role in cerebellar granule cell apoptosis, likely reducing the oxidative stress.",
keywords = "Apoptosis, Cerebellar granule cells, Fullerene derivative, Reactive oxygen species",
author = "M. Bisaglia and B. Natalini and R. Pellicciari and E. Straface and W. Malorni and D. Monti and C. Franceschi and G. Schettini",
year = "2000",
language = "English",
volume = "74",
pages = "1197--1204",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - C3-fullero-tris-methanodicarboxylic acid protects cerebellar granule cells from apoptosis

AU - Bisaglia, M.

AU - Natalini, B.

AU - Pellicciari, R.

AU - Straface, E.

AU - Malorni, W.

AU - Monti, D.

AU - Franceschi, C.

AU - Schettini, G.

PY - 2000

Y1 - 2000

N2 - Buckminsterfullerenols were recently investigated for their protective properties in different models of acute and chronic neurodegeneration. We tested C3-fullero-tris-methanodicarboxylic acid in our in vitro model of apoptotic neuronal death, which consists of shifting the culture K+ concentration from 25 to 5 mM for rat cerebellar granule cells. The impairment of mitochondrial respiratory function as well as chromatin derangement and fragmentation of DNA in apoptotic oligonucleosomes that occur in these conditions were protected by this compound in a concentration- dependent way. To assess whether antioxidant activity could account for the rescue of cerebellar granule cells from apoptosis, we tested the fullerene derivative under FeSO4-induced oxidative stress and found significant protection. Thus, we visualized membrane and cytoplasmic peroxides and reactive oxygen species and found a significant reduction of the species after 24 h in 5 mM K+ with the fullerene derivative. Such evidence suggests that this compound exerts a protective role in cerebellar granule cell apoptosis, likely reducing the oxidative stress.

AB - Buckminsterfullerenols were recently investigated for their protective properties in different models of acute and chronic neurodegeneration. We tested C3-fullero-tris-methanodicarboxylic acid in our in vitro model of apoptotic neuronal death, which consists of shifting the culture K+ concentration from 25 to 5 mM for rat cerebellar granule cells. The impairment of mitochondrial respiratory function as well as chromatin derangement and fragmentation of DNA in apoptotic oligonucleosomes that occur in these conditions were protected by this compound in a concentration- dependent way. To assess whether antioxidant activity could account for the rescue of cerebellar granule cells from apoptosis, we tested the fullerene derivative under FeSO4-induced oxidative stress and found significant protection. Thus, we visualized membrane and cytoplasmic peroxides and reactive oxygen species and found a significant reduction of the species after 24 h in 5 mM K+ with the fullerene derivative. Such evidence suggests that this compound exerts a protective role in cerebellar granule cell apoptosis, likely reducing the oxidative stress.

KW - Apoptosis

KW - Cerebellar granule cells

KW - Fullerene derivative

KW - Reactive oxygen species

UR - http://www.scopus.com/inward/record.url?scp=0033621949&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033621949&partnerID=8YFLogxK

M3 - Article

C2 - 10693952

AN - SCOPUS:0033621949

VL - 74

SP - 1197

EP - 1204

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 3

ER -