C3-fullero-tris-methanodicarboxylic acid protects cerebellar granule cells from apoptosis

M. Bisaglia, B. Natalini, R. Pellicciari, E. Straface, W. Malorni, D. Monti, C. Franceschi, G. Schettini

Research output: Contribution to journalArticle

Abstract

Buckminsterfullerenols were recently investigated for their protective properties in different models of acute and chronic neurodegeneration. We tested C3-fullero-tris-methanodicarboxylic acid in our in vitro model of apoptotic neuronal death, which consists of shifting the culture K+ concentration from 25 to 5 mM for rat cerebellar granule cells. The impairment of mitochondrial respiratory function as well as chromatin derangement and fragmentation of DNA in apoptotic oligonucleosomes that occur in these conditions were protected by this compound in a concentration- dependent way. To assess whether antioxidant activity could account for the rescue of cerebellar granule cells from apoptosis, we tested the fullerene derivative under FeSO4-induced oxidative stress and found significant protection. Thus, we visualized membrane and cytoplasmic peroxides and reactive oxygen species and found a significant reduction of the species after 24 h in 5 mM K+ with the fullerene derivative. Such evidence suggests that this compound exerts a protective role in cerebellar granule cell apoptosis, likely reducing the oxidative stress.

Original languageEnglish
Pages (from-to)1197-1204
Number of pages8
JournalJournal of Neurochemistry
Volume74
Issue number3
Publication statusPublished - 2000

Keywords

  • Apoptosis
  • Cerebellar granule cells
  • Fullerene derivative
  • Reactive oxygen species

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'C<sub>3</sub>-fullero-tris-methanodicarboxylic acid protects cerebellar granule cells from apoptosis'. Together they form a unique fingerprint.

  • Cite this

    Bisaglia, M., Natalini, B., Pellicciari, R., Straface, E., Malorni, W., Monti, D., Franceschi, C., & Schettini, G. (2000). C3-fullero-tris-methanodicarboxylic acid protects cerebellar granule cells from apoptosis. Journal of Neurochemistry, 74(3), 1197-1204.