CTAB-urea method purifies RNA from melanin for cDNA microarray analysis

M. Stefania Lagonigro, Loris De Cecco, Piero Carninci, Delia Di Stasi, Tiziana Ranzani, Monica Rodolfo, Manuela Gariboldi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Melanin represents a major problem for the study of melanoma by microarrays since it is retained during RNA extraction and inhibits the enzymatic reactions used for probe preparation. Here we report a new method for cleaning RNA from melanin, based on the use of the cationic detergent cetyl-trimethylammonium bromide (CTAB)-urea for RNA precipitation. This method is easy to perform and has a low cost. Purified RNA is recovered with high quality and good yield. CTAB-urea treated RNA from highly pigmented melanoma cells can be successfully reverse transcribed and labeled to obtain probes which can be subsequently used in cDNA microarray experiments, giving consistent and reproducible results.

Original languageEnglish
Pages (from-to)312-315
Number of pages4
JournalPigment Cell Research
Volume17
Issue number3
DOIs
Publication statusPublished - Jun 2004

Fingerprint

Melanins
melanin
Microarray Analysis
Microarrays
Oligonucleotide Array Sequence Analysis
bromides
Bromides
Urea
Complementary DNA
urea
RNA
melanoma
probes (equipment)
Melanoma
methodology
enzymatic reactions
detergents
cleaning
Detergents
Cleaning

Keywords

  • cDNA arrays
  • Melanin
  • Melanoma
  • Method
  • RNA purification

ASJC Scopus subject areas

  • Cell Biology
  • Agronomy and Crop Science
  • Plant Science
  • Clinical Biochemistry
  • Developmental Biology

Cite this

CTAB-urea method purifies RNA from melanin for cDNA microarray analysis. / Lagonigro, M. Stefania; De Cecco, Loris; Carninci, Piero; Di Stasi, Delia; Ranzani, Tiziana; Rodolfo, Monica; Gariboldi, Manuela.

In: Pigment Cell Research, Vol. 17, No. 3, 06.2004, p. 312-315.

Research output: Contribution to journalArticle

Lagonigro, M. Stefania ; De Cecco, Loris ; Carninci, Piero ; Di Stasi, Delia ; Ranzani, Tiziana ; Rodolfo, Monica ; Gariboldi, Manuela. / CTAB-urea method purifies RNA from melanin for cDNA microarray analysis. In: Pigment Cell Research. 2004 ; Vol. 17, No. 3. pp. 312-315.
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