CTLA-4 expressed by human dendritic cells modulates their cytokine secretion and induction of T cell proliferation

S. Laurent, P. Carrega, D. Saverino, P. Piccioli, M. Camoriano, A. Morabito, B. Dozin, V. Fontana, R. Simone, L. Mortara, M. C. Mingari, G. Ferlazzo, M. P. Pistillo

Research output: Contribution to journalArticle

Abstract

CTLA-4 is the major nefative regulator of T cell response. We have analyzed the expression of CTLA-4 in human monocytes and monocyte-derived DCs and the effects of its engagement on cytokine production and T cell stimulatory activity by mature DCs (mDCs). We found the CTLA-4 was highly expressed on freshly isolated monocytes, then down-modulated on the immature DCs (iDCs) and upregulated on mDCs. Treatment of mDCS with an agonistic anti-CTLA-4 m Ab enhanced secretion of IL-10 but reduced secretion of IL-8 and IL-12, as well as autologous CD4+ T-cell proliferation in response to simulation with PPD recall antigenloaded-DCs. Neutralization of IL-10 with an anti-IL-10 antibody partially restored the ability of anti-CTLA-4-treated mDCs to stimulate T cell proliferation in response to PPD. Our data provide the first evidence that CTLA-4 receptor is expressed by human mDCs and exerts immune modulatory effects in these cells.

Original languageEnglish
Pages (from-to)141-143
Number of pages3
JournalJournal of Biological Research (Italy)
Volume84
Issue number1
DOIs
Publication statusPublished - Jan 1 2011

Keywords

  • CTLA-4
  • Cytokine secretion
  • DC
  • T cell proliferation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Plant Science
  • Biochemistry, medical

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