CTLA4-Ig influenza e inibisce le attività proinfiammatorie di macrofagi sinoviali reumatoidi in monocoltura

Translated title of the contribution: CTLA4-Ig interferes and downregulates the proinflammatory activities of rheumatoid synovial macrophages in monoculture

R. Brizzolara, S. Soldano, P. Montagna, A. Sulli, B. Seriolo, B. Villaggio, P. F. Triolo, P. Clerico, L. Felli, L. Molfetta, M. Cutolo

Research output: Contribution to journalArticle

Abstract

Objective: CTLA4-Ig, a biologic agent employed in rheumatoid arthritis (RA) treatment, downregulates the immune response and exerts anti-inflammatory effects acting on different cells including dendritic/T cells interaction and directly on osteoclasts. We investigated the anti-inflammatory effects of CTLA4-Ig in primary monocultures of RA synovial macrophages (SM). Methods SM were obtained, from 8 RA patients (7 F, 1 M; DAS28>5.2) who underwent therapeutic arthroscopic synoviectomy and were cultured in the absence and in the presence of CTLA4-Ig at the concentration of [500 μg/ml], the most reliable dose related to the previous pharmacological clinical and experimental experiences. Inflammatory cytokine (IL-6, TNFalpha, IL-1beta) expression was evaluated by immunocytochemistry (ICC with relative image analysis), western blot (WB), and quantitative real-time polymerase chain reaction (qRT-PCR). Results: ICC analysis revealed that CTLA4-Ig treatment significantly downregulated cytokine expression (p

Translated title of the contributionCTLA4-Ig interferes and downregulates the proinflammatory activities of rheumatoid synovial macrophages in monoculture
Original languageItalian
Pages (from-to)80-85
Number of pages6
JournalReumatismo
Volume63
Issue number2
Publication statusPublished - 2011

ASJC Scopus subject areas

  • Rheumatology

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    Brizzolara, R., Soldano, S., Montagna, P., Sulli, A., Seriolo, B., Villaggio, B., Triolo, P. F., Clerico, P., Felli, L., Molfetta, L., & Cutolo, M. (2011). CTLA4-Ig influenza e inibisce le attività proinfiammatorie di macrofagi sinoviali reumatoidi in monocoltura. Reumatismo, 63(2), 80-85.