CTR2 identifies a population of cancer cells with stem cell-like features in patients with clear cell renal cell carcinoma

Vanessa Galleggiante, Monica Rutigliano, Fabio Sallustio, Domenico Ribatti, Pasquale Ditonno, Carlo Bettocchi, Francesco Paolo Selvaggi, Giuseppe Lucarelli, Michele Battaglia

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Abstract

Purpose In clear cell renal cell carcinoma tissue samples we identified and characterized a population of renal cell carcinoma derived CD133+/CD24+ cancer cells. We studied differences between these cells and their nonneoplastic counterpart, tubular adult renal progenitor cells.

Materials and Methods CD133+/CD24+ renal cell carcinoma derived cells were isolated from 40 patients. The mesenchymal phenotype and stemness proteomic profile of these renal cell carcinoma derived cells were characterized. Colony forming efficiency and self-renewal ability were tested by limiting dilution. Tumorigenic properties were evaluated in vitro by soft agar assay. The angiogenic response was evaluated in vivo by the chorioallantoic membrane angiogenic assay. Microarray analysis was performed on 6 tubular adult renal progenitor cell and 6 renal cell carcinoma derived cell clones. Membrane protein expression was evaluated by flow cytometry and immunofluorescence staining.

Results CD133+/CD24+ cells were isolated from normal and tumor kidney tissue. Fluorescence activated cell sorting revealed that renal cell carcinoma derived cells did not express mesenchymal stem cell markers. CD133+/CD24+ tumor cells were more undifferentiated than tubular adult renal progenitor cells. Renal cell carcinoma derived cells were clonigenic and could differentiate into adipocytes, epithelial and osteogenic cells. They could also regenerate tumor cells in vitro and induce angiogenesis in vivo. Gene expression profile identified CTR2 as a membrane marker for this neoplastic population. CTR2 was involved in renal cell carcinoma derived cell cisplatin resistance.

Conclusions Our results indicate the presence of a CD133+/CD24+/CTR2+ cancer cell population in clear cell renal cell carcinoma. These cells have some stem cell-like features, including in vitro self-maintenance and differentiating capabilities, and they can induce an angiogenic response in vivo.

Original languageEnglish
Pages (from-to)1831-1841
Number of pages11
JournalJournal of Urology
Volume192
Issue number6
DOIs
Publication statusPublished - Dec 1 2014

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Renal Cell Carcinoma
Stem Cells
Population
Neoplasms
Kidney
Flow Cytometry
Chorioallantoic Membrane
Microarray Analysis
Mesenchymal Stromal Cells
Transcriptome
Adipocytes
Proteomics
Cisplatin
Agar
Fluorescent Antibody Technique
Membrane Proteins
Clone Cells
Epithelial Cells
Staining and Labeling
Phenotype

Keywords

  • AC133 antigen
  • antigens
  • carcinoma
  • CD24
  • CTR2 protein
  • human
  • kidney
  • renal cell

ASJC Scopus subject areas

  • Urology
  • Medicine(all)

Cite this

Galleggiante, V., Rutigliano, M., Sallustio, F., Ribatti, D., Ditonno, P., Bettocchi, C., ... Battaglia, M. (2014). CTR2 identifies a population of cancer cells with stem cell-like features in patients with clear cell renal cell carcinoma. Journal of Urology, 192(6), 1831-1841. https://doi.org/10.1016/j.juro.2014.06.070

CTR2 identifies a population of cancer cells with stem cell-like features in patients with clear cell renal cell carcinoma. / Galleggiante, Vanessa; Rutigliano, Monica; Sallustio, Fabio; Ribatti, Domenico; Ditonno, Pasquale; Bettocchi, Carlo; Selvaggi, Francesco Paolo; Lucarelli, Giuseppe; Battaglia, Michele.

In: Journal of Urology, Vol. 192, No. 6, 01.12.2014, p. 1831-1841.

Research output: Contribution to journalArticle

Galleggiante, V, Rutigliano, M, Sallustio, F, Ribatti, D, Ditonno, P, Bettocchi, C, Selvaggi, FP, Lucarelli, G & Battaglia, M 2014, 'CTR2 identifies a population of cancer cells with stem cell-like features in patients with clear cell renal cell carcinoma', Journal of Urology, vol. 192, no. 6, pp. 1831-1841. https://doi.org/10.1016/j.juro.2014.06.070
Galleggiante, Vanessa ; Rutigliano, Monica ; Sallustio, Fabio ; Ribatti, Domenico ; Ditonno, Pasquale ; Bettocchi, Carlo ; Selvaggi, Francesco Paolo ; Lucarelli, Giuseppe ; Battaglia, Michele. / CTR2 identifies a population of cancer cells with stem cell-like features in patients with clear cell renal cell carcinoma. In: Journal of Urology. 2014 ; Vol. 192, No. 6. pp. 1831-1841.
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abstract = "Purpose In clear cell renal cell carcinoma tissue samples we identified and characterized a population of renal cell carcinoma derived CD133+/CD24+ cancer cells. We studied differences between these cells and their nonneoplastic counterpart, tubular adult renal progenitor cells.Materials and Methods CD133+/CD24+ renal cell carcinoma derived cells were isolated from 40 patients. The mesenchymal phenotype and stemness proteomic profile of these renal cell carcinoma derived cells were characterized. Colony forming efficiency and self-renewal ability were tested by limiting dilution. Tumorigenic properties were evaluated in vitro by soft agar assay. The angiogenic response was evaluated in vivo by the chorioallantoic membrane angiogenic assay. Microarray analysis was performed on 6 tubular adult renal progenitor cell and 6 renal cell carcinoma derived cell clones. Membrane protein expression was evaluated by flow cytometry and immunofluorescence staining.Results CD133+/CD24+ cells were isolated from normal and tumor kidney tissue. Fluorescence activated cell sorting revealed that renal cell carcinoma derived cells did not express mesenchymal stem cell markers. CD133+/CD24+ tumor cells were more undifferentiated than tubular adult renal progenitor cells. Renal cell carcinoma derived cells were clonigenic and could differentiate into adipocytes, epithelial and osteogenic cells. They could also regenerate tumor cells in vitro and induce angiogenesis in vivo. Gene expression profile identified CTR2 as a membrane marker for this neoplastic population. CTR2 was involved in renal cell carcinoma derived cell cisplatin resistance.Conclusions Our results indicate the presence of a CD133+/CD24+/CTR2+ cancer cell population in clear cell renal cell carcinoma. These cells have some stem cell-like features, including in vitro self-maintenance and differentiating capabilities, and they can induce an angiogenic response in vivo.",
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T1 - CTR2 identifies a population of cancer cells with stem cell-like features in patients with clear cell renal cell carcinoma

AU - Galleggiante, Vanessa

AU - Rutigliano, Monica

AU - Sallustio, Fabio

AU - Ribatti, Domenico

AU - Ditonno, Pasquale

AU - Bettocchi, Carlo

AU - Selvaggi, Francesco Paolo

AU - Lucarelli, Giuseppe

AU - Battaglia, Michele

PY - 2014/12/1

Y1 - 2014/12/1

N2 - Purpose In clear cell renal cell carcinoma tissue samples we identified and characterized a population of renal cell carcinoma derived CD133+/CD24+ cancer cells. We studied differences between these cells and their nonneoplastic counterpart, tubular adult renal progenitor cells.Materials and Methods CD133+/CD24+ renal cell carcinoma derived cells were isolated from 40 patients. The mesenchymal phenotype and stemness proteomic profile of these renal cell carcinoma derived cells were characterized. Colony forming efficiency and self-renewal ability were tested by limiting dilution. Tumorigenic properties were evaluated in vitro by soft agar assay. The angiogenic response was evaluated in vivo by the chorioallantoic membrane angiogenic assay. Microarray analysis was performed on 6 tubular adult renal progenitor cell and 6 renal cell carcinoma derived cell clones. Membrane protein expression was evaluated by flow cytometry and immunofluorescence staining.Results CD133+/CD24+ cells were isolated from normal and tumor kidney tissue. Fluorescence activated cell sorting revealed that renal cell carcinoma derived cells did not express mesenchymal stem cell markers. CD133+/CD24+ tumor cells were more undifferentiated than tubular adult renal progenitor cells. Renal cell carcinoma derived cells were clonigenic and could differentiate into adipocytes, epithelial and osteogenic cells. They could also regenerate tumor cells in vitro and induce angiogenesis in vivo. Gene expression profile identified CTR2 as a membrane marker for this neoplastic population. CTR2 was involved in renal cell carcinoma derived cell cisplatin resistance.Conclusions Our results indicate the presence of a CD133+/CD24+/CTR2+ cancer cell population in clear cell renal cell carcinoma. These cells have some stem cell-like features, including in vitro self-maintenance and differentiating capabilities, and they can induce an angiogenic response in vivo.

AB - Purpose In clear cell renal cell carcinoma tissue samples we identified and characterized a population of renal cell carcinoma derived CD133+/CD24+ cancer cells. We studied differences between these cells and their nonneoplastic counterpart, tubular adult renal progenitor cells.Materials and Methods CD133+/CD24+ renal cell carcinoma derived cells were isolated from 40 patients. The mesenchymal phenotype and stemness proteomic profile of these renal cell carcinoma derived cells were characterized. Colony forming efficiency and self-renewal ability were tested by limiting dilution. Tumorigenic properties were evaluated in vitro by soft agar assay. The angiogenic response was evaluated in vivo by the chorioallantoic membrane angiogenic assay. Microarray analysis was performed on 6 tubular adult renal progenitor cell and 6 renal cell carcinoma derived cell clones. Membrane protein expression was evaluated by flow cytometry and immunofluorescence staining.Results CD133+/CD24+ cells were isolated from normal and tumor kidney tissue. Fluorescence activated cell sorting revealed that renal cell carcinoma derived cells did not express mesenchymal stem cell markers. CD133+/CD24+ tumor cells were more undifferentiated than tubular adult renal progenitor cells. Renal cell carcinoma derived cells were clonigenic and could differentiate into adipocytes, epithelial and osteogenic cells. They could also regenerate tumor cells in vitro and induce angiogenesis in vivo. Gene expression profile identified CTR2 as a membrane marker for this neoplastic population. CTR2 was involved in renal cell carcinoma derived cell cisplatin resistance.Conclusions Our results indicate the presence of a CD133+/CD24+/CTR2+ cancer cell population in clear cell renal cell carcinoma. These cells have some stem cell-like features, including in vitro self-maintenance and differentiating capabilities, and they can induce an angiogenic response in vivo.

KW - AC133 antigen

KW - antigens

KW - carcinoma

KW - CD24

KW - CTR2 protein

KW - human

KW - kidney

KW - renal cell

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