Cubosomes for in vivo fluorescence lifetime imaging

Stefania Biffi, Laura Andolfi, Claudia Caltagirone, Chiara Garrovo, Angela M. Falchi, Vito Lippolis, Andrea Lorenzon, Paolo Macor, Valeria Meli, Maura Monduzzi, Marc Obiols-Rabasa, Luca Petrizza, Luca Prodi, Antonella de Rosa, Judith Schmidt, Yeshayahu Talmon, Sergio Murgia

Research output: Contribution to journalArticlepeer-review


Herein we provided the first proof of principle for in vivo fluorescence optical imaging application using monoolein-based cubosomes in a healthy mouse animal model. This formulation, administered at a non-cytotoxic concentration, was capable of providing both exogenous contrast for NIR fluorescence imaging with very high efficiency and chemospecific information upon lifetime analysis. Time-resolved measurements of fluorescence after the intravenous injection of cubosomes revealed that the dye rapidly accumulated mainly in the liver, while lifetimes profiles obtained in vivo allowed for discriminating between free dye or dye embedded within the cubosome nanostructure after injection.

Original languageEnglish
Article number055102
Issue number5
Publication statusPublished - Feb 3 2017


  • fluorescence lifetime probe
  • fluorescence optical imaging in vivo
  • liquid crystalline nanoparticles

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering


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