Cubosomes for in vivo fluorescence lifetime imaging

Stefania Biffi, Laura Andolfi, Claudia Caltagirone, Chiara Garrovo, Angela M. Falchi, Vito Lippolis, Andrea Lorenzon, Paolo Macor, Valeria Meli, Maura Monduzzi, Marc Obiols-Rabasa, Luca Petrizza, Luca Prodi, Antonella de Rosa, Judith Schmidt, Yeshayahu Talmon, Sergio Murgia

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Herein we provided the first proof of principle for in vivo fluorescence optical imaging application using monoolein-based cubosomes in a healthy mouse animal model. This formulation, administered at a non-cytotoxic concentration, was capable of providing both exogenous contrast for NIR fluorescence imaging with very high efficiency and chemospecific information upon lifetime analysis. Time-resolved measurements of fluorescence after the intravenous injection of cubosomes revealed that the dye rapidly accumulated mainly in the liver, while lifetimes profiles obtained in vivo allowed for discriminating between free dye or dye embedded within the cubosome nanostructure after injection.

Original languageEnglish
Article number055102
JournalNanotechnology
Volume28
Issue number5
DOIs
Publication statusPublished - Feb 3 2017

Fingerprint

Coloring Agents
Dyes
Fluorescence
Imaging techniques
Time measurement
Liver
Nanostructures
Animals
monoolein

Keywords

  • fluorescence lifetime probe
  • fluorescence optical imaging in vivo
  • liquid crystalline nanoparticles

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Biffi, S., Andolfi, L., Caltagirone, C., Garrovo, C., Falchi, A. M., Lippolis, V., ... Murgia, S. (2017). Cubosomes for in vivo fluorescence lifetime imaging. Nanotechnology, 28(5), [055102]. https://doi.org/10.1088/1361-6528/28/5/055102

Cubosomes for in vivo fluorescence lifetime imaging. / Biffi, Stefania; Andolfi, Laura; Caltagirone, Claudia; Garrovo, Chiara; Falchi, Angela M.; Lippolis, Vito; Lorenzon, Andrea; Macor, Paolo; Meli, Valeria; Monduzzi, Maura; Obiols-Rabasa, Marc; Petrizza, Luca; Prodi, Luca; de Rosa, Antonella; Schmidt, Judith; Talmon, Yeshayahu; Murgia, Sergio.

In: Nanotechnology, Vol. 28, No. 5, 055102, 03.02.2017.

Research output: Contribution to journalArticle

Biffi, S, Andolfi, L, Caltagirone, C, Garrovo, C, Falchi, AM, Lippolis, V, Lorenzon, A, Macor, P, Meli, V, Monduzzi, M, Obiols-Rabasa, M, Petrizza, L, Prodi, L, de Rosa, A, Schmidt, J, Talmon, Y & Murgia, S 2017, 'Cubosomes for in vivo fluorescence lifetime imaging', Nanotechnology, vol. 28, no. 5, 055102. https://doi.org/10.1088/1361-6528/28/5/055102
Biffi S, Andolfi L, Caltagirone C, Garrovo C, Falchi AM, Lippolis V et al. Cubosomes for in vivo fluorescence lifetime imaging. Nanotechnology. 2017 Feb 3;28(5). 055102. https://doi.org/10.1088/1361-6528/28/5/055102
Biffi, Stefania ; Andolfi, Laura ; Caltagirone, Claudia ; Garrovo, Chiara ; Falchi, Angela M. ; Lippolis, Vito ; Lorenzon, Andrea ; Macor, Paolo ; Meli, Valeria ; Monduzzi, Maura ; Obiols-Rabasa, Marc ; Petrizza, Luca ; Prodi, Luca ; de Rosa, Antonella ; Schmidt, Judith ; Talmon, Yeshayahu ; Murgia, Sergio. / Cubosomes for in vivo fluorescence lifetime imaging. In: Nanotechnology. 2017 ; Vol. 28, No. 5.
@article{39ae5f25ba9a442dbbe1db290a7afd11,
title = "Cubosomes for in vivo fluorescence lifetime imaging",
abstract = "Herein we provided the first proof of principle for in vivo fluorescence optical imaging application using monoolein-based cubosomes in a healthy mouse animal model. This formulation, administered at a non-cytotoxic concentration, was capable of providing both exogenous contrast for NIR fluorescence imaging with very high efficiency and chemospecific information upon lifetime analysis. Time-resolved measurements of fluorescence after the intravenous injection of cubosomes revealed that the dye rapidly accumulated mainly in the liver, while lifetimes profiles obtained in vivo allowed for discriminating between free dye or dye embedded within the cubosome nanostructure after injection.",
keywords = "fluorescence lifetime probe, fluorescence optical imaging in vivo, liquid crystalline nanoparticles",
author = "Stefania Biffi and Laura Andolfi and Claudia Caltagirone and Chiara Garrovo and Falchi, {Angela M.} and Vito Lippolis and Andrea Lorenzon and Paolo Macor and Valeria Meli and Maura Monduzzi and Marc Obiols-Rabasa and Luca Petrizza and Luca Prodi and {de Rosa}, Antonella and Judith Schmidt and Yeshayahu Talmon and Sergio Murgia",
year = "2017",
month = "2",
day = "3",
doi = "10.1088/1361-6528/28/5/055102",
language = "English",
volume = "28",
journal = "Nanotechnology",
issn = "0957-4484",
publisher = "IOP Publishing Ltd.",
number = "5",

}

TY - JOUR

T1 - Cubosomes for in vivo fluorescence lifetime imaging

AU - Biffi, Stefania

AU - Andolfi, Laura

AU - Caltagirone, Claudia

AU - Garrovo, Chiara

AU - Falchi, Angela M.

AU - Lippolis, Vito

AU - Lorenzon, Andrea

AU - Macor, Paolo

AU - Meli, Valeria

AU - Monduzzi, Maura

AU - Obiols-Rabasa, Marc

AU - Petrizza, Luca

AU - Prodi, Luca

AU - de Rosa, Antonella

AU - Schmidt, Judith

AU - Talmon, Yeshayahu

AU - Murgia, Sergio

PY - 2017/2/3

Y1 - 2017/2/3

N2 - Herein we provided the first proof of principle for in vivo fluorescence optical imaging application using monoolein-based cubosomes in a healthy mouse animal model. This formulation, administered at a non-cytotoxic concentration, was capable of providing both exogenous contrast for NIR fluorescence imaging with very high efficiency and chemospecific information upon lifetime analysis. Time-resolved measurements of fluorescence after the intravenous injection of cubosomes revealed that the dye rapidly accumulated mainly in the liver, while lifetimes profiles obtained in vivo allowed for discriminating between free dye or dye embedded within the cubosome nanostructure after injection.

AB - Herein we provided the first proof of principle for in vivo fluorescence optical imaging application using monoolein-based cubosomes in a healthy mouse animal model. This formulation, administered at a non-cytotoxic concentration, was capable of providing both exogenous contrast for NIR fluorescence imaging with very high efficiency and chemospecific information upon lifetime analysis. Time-resolved measurements of fluorescence after the intravenous injection of cubosomes revealed that the dye rapidly accumulated mainly in the liver, while lifetimes profiles obtained in vivo allowed for discriminating between free dye or dye embedded within the cubosome nanostructure after injection.

KW - fluorescence lifetime probe

KW - fluorescence optical imaging in vivo

KW - liquid crystalline nanoparticles

UR - http://www.scopus.com/inward/record.url?scp=85009113821&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85009113821&partnerID=8YFLogxK

U2 - 10.1088/1361-6528/28/5/055102

DO - 10.1088/1361-6528/28/5/055102

M3 - Article

VL - 28

JO - Nanotechnology

JF - Nanotechnology

SN - 0957-4484

IS - 5

M1 - 055102

ER -