Cu(II) and Zn(II) complexes with hyaluronic acid and its sulphated derivative. Effect on the motility of vascular endothelial cells

Rolando Barbucci, Agnese Magnani, Stefania Lamponi, Stefania Mitola, Marina Ziche, Lucia Morbidelli, Federico Bussolino

Research output: Contribution to journalArticlepeer-review

Abstract

With the aim of improving the compatibility of biomaterials to be used for the construction of cardiovascular prosthesis, we have designed bioactive macromolecules resulting from chemical modifications of hyaluronic acid (Hyal). The stability constants of Cu(II) and Zn(II) complexes with the sulphated derivative of hyaluronic acid (HyalS3.5) were evaluated. Two different complexes have been found for each metal ion, CuL, Cu(OH)2L and ZnL, Zn(OH)2L (L means the disaccharide unit of the ligands) in aqueous solution at 37°C. The dihydroxo Cu(II) complex was present in high percentage at pH=7.4. On the contrary, the Zn(II) ion was present with a relatively low percentage of both complexes. The ability to stimulate endothelial cell adhesion and migration was evaluated for Hyal, HyalS3.5 and their complexes with Cu(II) and Zn(II) ions. The results revealed that Hyal and [Cu(OH)2HyalS3.5]((4.5)-) induced cell adhesion, while [ZnHyalS3.5]((2.5)-) and [Zn(OH)2HyalS3.5]((4.5)-) inhibited the process. The chemotactic activity of increasing concentrations of the above complexes was also evaluated, demonstrating that [Cu(OH)2HyalS3.5]((4.5)-) complex at 1 μM concentration was the most active in inducing cell migration. These results have been also strengthened by analysing adherent cell migration in agarose. In conclusion, sulphated hyaluronic acid coordinated to Cu(II) seems to be a promising matrix molecule for the construction of cardiovascular prosthesis. Copyright (C) 2000 Elsevier Science S.A.

Original languageEnglish
Pages (from-to)229-237
Number of pages9
JournalJournal of Inorganic Biochemistry
Volume81
Issue number4
DOIs
Publication statusPublished - Oct 1 2000

Keywords

  • Adhesion
  • Chemotaxis
  • Cu(II) and Zn(II) complexes
  • Endothelial cells
  • Metal ion complexes
  • Migration
  • Sulphated hyaluronic acid

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

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