Abstract
Cultured human keratinocytes have a wide spectrum of clinical applications. Clinical results reported by several investigators are, however, contradictory. In this review, the authors discuss the biological and surgical issues which play a key role in the clinical outcome of cultured epidermal autografts used for the treatment of massive full-thickness burns. The importance of cultivation of epidermal stem cells and of their transplantation onto a wound bed prepared with donor dermis is emphasised. The paper also reviews recent data showing that: (i) cultured epidermal autografts bearing melanocytes can be used for the treatment of stable vitiligo; (ii) keratinocytes isolated from other lining epithelia, such as oral, urethral and corneal epithelia, can be cultivated and grafted onto patients suffering from disabling epithelial defects; (iii) keratinocyte stem cells can be stably transduced with retroviral vectors and are therefore attractive targets for the gene therapy of genodermatoses.
| Original language | English |
|---|---|
| Pages (from-to) | 778-790 |
| Number of pages | 13 |
| Journal | Medical and Biological Engineering and Computing |
| Volume | 36 |
| Issue number | 6 |
| Publication status | Published - Nov 1998 |
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Keywords
- Burns
- Epidermis
- Gene therapy
- Genodermatoses
- Keratinocyte
- Melanocyte
- Skin
- Stem cell
- Tissue engineering
- Vitiligo
ASJC Scopus subject areas
- Biomedical Engineering
- Health Informatics
- Health Information Management
- Computer Science Applications
- Computational Theory and Mathematics
Cite this
Cultivation of human keratinocyte stem cells : Current and future clinical applications. / Pellegrini, G.; Bondanza, S.; Guerra, L.; De Luca, M.
In: Medical and Biological Engineering and Computing, Vol. 36, No. 6, 11.1998, p. 778-790.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cultivation of human keratinocyte stem cells
T2 - Current and future clinical applications
AU - Pellegrini, G.
AU - Bondanza, S.
AU - Guerra, L.
AU - De Luca, M.
PY - 1998/11
Y1 - 1998/11
N2 - Cultured human keratinocytes have a wide spectrum of clinical applications. Clinical results reported by several investigators are, however, contradictory. In this review, the authors discuss the biological and surgical issues which play a key role in the clinical outcome of cultured epidermal autografts used for the treatment of massive full-thickness burns. The importance of cultivation of epidermal stem cells and of their transplantation onto a wound bed prepared with donor dermis is emphasised. The paper also reviews recent data showing that: (i) cultured epidermal autografts bearing melanocytes can be used for the treatment of stable vitiligo; (ii) keratinocytes isolated from other lining epithelia, such as oral, urethral and corneal epithelia, can be cultivated and grafted onto patients suffering from disabling epithelial defects; (iii) keratinocyte stem cells can be stably transduced with retroviral vectors and are therefore attractive targets for the gene therapy of genodermatoses.
AB - Cultured human keratinocytes have a wide spectrum of clinical applications. Clinical results reported by several investigators are, however, contradictory. In this review, the authors discuss the biological and surgical issues which play a key role in the clinical outcome of cultured epidermal autografts used for the treatment of massive full-thickness burns. The importance of cultivation of epidermal stem cells and of their transplantation onto a wound bed prepared with donor dermis is emphasised. The paper also reviews recent data showing that: (i) cultured epidermal autografts bearing melanocytes can be used for the treatment of stable vitiligo; (ii) keratinocytes isolated from other lining epithelia, such as oral, urethral and corneal epithelia, can be cultivated and grafted onto patients suffering from disabling epithelial defects; (iii) keratinocyte stem cells can be stably transduced with retroviral vectors and are therefore attractive targets for the gene therapy of genodermatoses.
KW - Burns
KW - Epidermis
KW - Gene therapy
KW - Genodermatoses
KW - Keratinocyte
KW - Melanocyte
KW - Skin
KW - Stem cell
KW - Tissue engineering
KW - Vitiligo
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UR - http://www.scopus.com/inward/citedby.url?scp=0032213367&partnerID=8YFLogxK
M3 - Article
VL - 36
SP - 778
EP - 790
JO - Medical and Biological Engineering and Computing
JF - Medical and Biological Engineering and Computing
SN - 0140-0118
IS - 6
ER -