Cultivation of human keratinocyte stem cells

Current and future clinical applications

G. Pellegrini, S. Bondanza, L. Guerra, M. De Luca

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Cultured human keratinocytes have a wide spectrum of clinical applications. Clinical results reported by several investigators are, however, contradictory. In this review, the authors discuss the biological and surgical issues which play a key role in the clinical outcome of cultured epidermal autografts used for the treatment of massive full-thickness burns. The importance of cultivation of epidermal stem cells and of their transplantation onto a wound bed prepared with donor dermis is emphasised. The paper also reviews recent data showing that: (i) cultured epidermal autografts bearing melanocytes can be used for the treatment of stable vitiligo; (ii) keratinocytes isolated from other lining epithelia, such as oral, urethral and corneal epithelia, can be cultivated and grafted onto patients suffering from disabling epithelial defects; (iii) keratinocyte stem cells can be stably transduced with retroviral vectors and are therefore attractive targets for the gene therapy of genodermatoses.

Original languageEnglish
Pages (from-to)778-790
Number of pages13
JournalMedical and Biological Engineering and Computing
Volume36
Issue number6
Publication statusPublished - Nov 1998

Fingerprint

Stem cells
Keratinocytes
Stem Cells
Autografts
Bearings (structural)
Gene therapy
Corneal Epithelium
Vitiligo
Melanocytes
Stem Cell Transplantation
Dermis
Burns
Linings
Genetic Therapy
Epithelium
Research Personnel
Tissue Donors
Defects
Wounds and Injuries
Therapeutics

Keywords

  • Burns
  • Epidermis
  • Gene therapy
  • Genodermatoses
  • Keratinocyte
  • Melanocyte
  • Skin
  • Stem cell
  • Tissue engineering
  • Vitiligo

ASJC Scopus subject areas

  • Biomedical Engineering
  • Health Informatics
  • Health Information Management
  • Computer Science Applications
  • Computational Theory and Mathematics

Cite this

Cultivation of human keratinocyte stem cells : Current and future clinical applications. / Pellegrini, G.; Bondanza, S.; Guerra, L.; De Luca, M.

In: Medical and Biological Engineering and Computing, Vol. 36, No. 6, 11.1998, p. 778-790.

Research output: Contribution to journalArticle

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