Culture conditions allow selection of different mesenchymal progenitors from adult mouse bone marrow.

Maria Teresa Esposito, Rosa Di Noto, Peppino Mirabelli, Marisa Gorrese, Silvia Parisi, Donatella Montanaro, Luigi Del Vecchio, Lucio Pastore

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Abstract

The use of adult stem cells in tissue engineering approaches will benefit from the establishment of culture conditions that allow the expansion and maintenance of cells with stem cell-like activity and high differentiation potential. In the field of adult stem cells, bone marrow stromal cells (BMSCs) are promising candidates. In the present study, we define, for the first time, conditions for optimizing the yields of cultures enriched for specific progenitors of bone marrow. Using four distinct culture conditions, supernatants from culture of bone fragments, marrow stroma cell line MS-5, embryonic fibroblast cell line NIH3T3, and a cocktail of epidermal growth factor (EGF) and platelet-derived growth factor (PDGF), we isolated four different sub-populations of murine BMSCs (mBMSCs). These cells express a well-known marker of undifferentiated embryonic stem cells (Nanog) and show interesting features in immunophenotype, self-renewal ability, and differentiation potency. In particular, using NIH3T3 conditioned medium, we obtained cells that showed impairment in osteogenic and chondrogenic differentiation while retaining high adipogenic potential during passages. Our results indicate that the choice of the medium used for isolation and expansion of mBMSCs is important for enriching the culture of desired specific progenitors.

Original languageEnglish
Pages (from-to)2525-2536
Number of pages12
JournalTissue Engineering - Part A
Volume15
Issue number9
DOIs
Publication statusPublished - Sep 2009

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ASJC Scopus subject areas

  • Medicine(all)

Cite this

Esposito, M. T., Di Noto, R., Mirabelli, P., Gorrese, M., Parisi, S., Montanaro, D., Del Vecchio, L., & Pastore, L. (2009). Culture conditions allow selection of different mesenchymal progenitors from adult mouse bone marrow. Tissue Engineering - Part A, 15(9), 2525-2536. https://doi.org/10.1089/ten.tea.2008.0509