Culture medium modulates proinflammatory conditions of human pancreatic islets before transplantation

S. Marzorati, B. Antonioli, R. Nano, P. Maffi, L. Piemonti, C. Giliola, A. Secchi, J. R. Lakey, F. Bertuzzi

Research output: Contribution to journalArticlepeer-review

Abstract

A portion of transplanted islets is lost during engraftment as a result of stressful events, involving hypoxia and production of proinflammatory molecules by islets. Two of these molecules (monocyte chemoattractant protein-1, CCL2/MCP-1 and tissue factor, TF) are directly correlated with reduced graft function. We evaluated which factors reduce islet proinflammatory conditions. In particular the effects of different culture media supplemented with proteins or antioxidant agents on CCL2/MCP-1 and TF human islet release were evaluated. We observed that human islets after culture in final wash culture medium (FW) significantly decreased CCL2/MCP-1 release and TF production compared with CMRL and M199. These effects were independent from the type of protein added to the media (human serum, human albumin, fetal calf serum). Glutathione in FW further decreased CCL2/MCP-1 in a dose-dependent manner. Culture conditions can modulate the proinflammatory state of islets, and could be used in clinical islet transplantation to reduce inflammation during engraftment.

Original languageEnglish
Pages (from-to)2791-2795
Number of pages5
JournalAmerican Journal of Transplantation
Volume6
Issue number11
DOIs
Publication statusPublished - Nov 2006

Keywords

  • Culture
  • Islet transplantation
  • Monocyte chemoattractant protein-1
  • Tissue factor

ASJC Scopus subject areas

  • Immunology

Fingerprint

Dive into the research topics of 'Culture medium modulates proinflammatory conditions of human pancreatic islets before transplantation'. Together they form a unique fingerprint.

Cite this