Culture of human endometrial cells

A new simple technique to completely separate epithelial glands

P. Vigano, A. M. Di Blasio, G. Dell' Antonio, M. Vignali

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

In vitro studies on dispersed human endometrial cells are still difficult to perform as the techniques employed do not allow an optimal separation of the cells. In particular, epithelial glands tend to maintain their tubular structure even after enzymatic dispersion. This could be a disadvantage when monolayers of single well dispersed cells are needed. In this paper we describe a new technique to establish monolayer cultures of isolated endometrial stromal and epithelial cell populations. After a first collagenase digestion, stromal and epithelial cells were separated by differential sedimentation at unity gravity. The epithelial glands obtained were further dispersed in single cells using a short incubation with low amount of trypsin. Morphologic characterization was performed using immunohistochemistry for vimentin and cytokeratins. Compared to previous described methods, this procedure is shorter and could better preserve cell surface structures. Thus, it could be successfully employed for in vitro studies of human endometrial pathophysiology.

Original languageEnglish
Pages (from-to)87-92
Number of pages6
JournalActa Obstetricia et Gynecologica Scandinavica
Volume72
Issue number2
Publication statusPublished - 1993

Fingerprint

Stromal Cells
Epithelial Cells
Cell Separation
Gravitation
Vimentin
Collagenases
Keratins
Trypsin
Digestion
Immunohistochemistry
Population
In Vitro Techniques

Keywords

  • Cytokeratin
  • Endometrium
  • Epithelial cells
  • Stromal cells
  • Vimentin

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Culture of human endometrial cells : A new simple technique to completely separate epithelial glands. / Vigano, P.; Di Blasio, A. M.; Dell' Antonio, G.; Vignali, M.

In: Acta Obstetricia et Gynecologica Scandinavica, Vol. 72, No. 2, 1993, p. 87-92.

Research output: Contribution to journalArticle

@article{77c69e2e268549df97f210b0cbc9985b,
title = "Culture of human endometrial cells: A new simple technique to completely separate epithelial glands",
abstract = "In vitro studies on dispersed human endometrial cells are still difficult to perform as the techniques employed do not allow an optimal separation of the cells. In particular, epithelial glands tend to maintain their tubular structure even after enzymatic dispersion. This could be a disadvantage when monolayers of single well dispersed cells are needed. In this paper we describe a new technique to establish monolayer cultures of isolated endometrial stromal and epithelial cell populations. After a first collagenase digestion, stromal and epithelial cells were separated by differential sedimentation at unity gravity. The epithelial glands obtained were further dispersed in single cells using a short incubation with low amount of trypsin. Morphologic characterization was performed using immunohistochemistry for vimentin and cytokeratins. Compared to previous described methods, this procedure is shorter and could better preserve cell surface structures. Thus, it could be successfully employed for in vitro studies of human endometrial pathophysiology.",
keywords = "Cytokeratin, Endometrium, Epithelial cells, Stromal cells, Vimentin",
author = "P. Vigano and {Di Blasio}, {A. M.} and {Dell' Antonio}, G. and M. Vignali",
year = "1993",
language = "English",
volume = "72",
pages = "87--92",
journal = "Acta Obstetricia et Gynecologica Scandinavica",
issn = "0001-6349",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Culture of human endometrial cells

T2 - A new simple technique to completely separate epithelial glands

AU - Vigano, P.

AU - Di Blasio, A. M.

AU - Dell' Antonio, G.

AU - Vignali, M.

PY - 1993

Y1 - 1993

N2 - In vitro studies on dispersed human endometrial cells are still difficult to perform as the techniques employed do not allow an optimal separation of the cells. In particular, epithelial glands tend to maintain their tubular structure even after enzymatic dispersion. This could be a disadvantage when monolayers of single well dispersed cells are needed. In this paper we describe a new technique to establish monolayer cultures of isolated endometrial stromal and epithelial cell populations. After a first collagenase digestion, stromal and epithelial cells were separated by differential sedimentation at unity gravity. The epithelial glands obtained were further dispersed in single cells using a short incubation with low amount of trypsin. Morphologic characterization was performed using immunohistochemistry for vimentin and cytokeratins. Compared to previous described methods, this procedure is shorter and could better preserve cell surface structures. Thus, it could be successfully employed for in vitro studies of human endometrial pathophysiology.

AB - In vitro studies on dispersed human endometrial cells are still difficult to perform as the techniques employed do not allow an optimal separation of the cells. In particular, epithelial glands tend to maintain their tubular structure even after enzymatic dispersion. This could be a disadvantage when monolayers of single well dispersed cells are needed. In this paper we describe a new technique to establish monolayer cultures of isolated endometrial stromal and epithelial cell populations. After a first collagenase digestion, stromal and epithelial cells were separated by differential sedimentation at unity gravity. The epithelial glands obtained were further dispersed in single cells using a short incubation with low amount of trypsin. Morphologic characterization was performed using immunohistochemistry for vimentin and cytokeratins. Compared to previous described methods, this procedure is shorter and could better preserve cell surface structures. Thus, it could be successfully employed for in vitro studies of human endometrial pathophysiology.

KW - Cytokeratin

KW - Endometrium

KW - Epithelial cells

KW - Stromal cells

KW - Vimentin

UR - http://www.scopus.com/inward/record.url?scp=0027417826&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027417826&partnerID=8YFLogxK

M3 - Article

VL - 72

SP - 87

EP - 92

JO - Acta Obstetricia et Gynecologica Scandinavica

JF - Acta Obstetricia et Gynecologica Scandinavica

SN - 0001-6349

IS - 2

ER -