Cultures of a human synovial cell line to evaluate platelet-rich plasma and hyaluronic acid effects

Eleonora Olivotto, Giulia Merli, Elisa Assirelli, Carola Cavallo, E Belluzzi, R Ramonda, M Favero, G Filardo, Alice Roffi, E Kon, Brunella Grigolo

Research output: Contribution to journalArticle

Abstract

Synovial inflammation plays an important role in osteoarthritis (OA) pathogenesis. Different biological compounds have been tested mainly on chondrocytes, to treat early stages of OA. However, since OA has been recently defined as "an organ" pathology, investigation on synoviocytes is also needed. Therefore the aim of the present study was to validate a human fibroblast-like synoviocytes cell line (K4IM) to test the effects of platelet-rich plasma (PRP) and hyaluronan (HA) on anabolic and catabolic gene expression and on HA secretion from cell cultures. In order to determine the effect of PRP and HA, K4IM cells were maintained in culture with or without TNF-α stimulation. In the presence of PRP, unstimulated K4IM cells presented the same expression of IL1B, IL6, CXCL8, VEGF, TIMP1 and hyaluronic synthase isoform HAS3 as primary human synoviocytes, while HA addition did not change their expression pattern, which was similar to control cells. Stimulated cells expressed significantly higher values of IL1B, CXCL8 and VEGF compared to unstimulated ones. PRP did not show any modification, except for VEGF, while HA addition modulated IL1B expression. PRP did not modulate HA release of both stimulated and unstimulated cells. Our study showed the possibility to use K4IM synoviocytes as an in vitro model to test biological compounds useful for the treatment of early OA. Primary cells reflect the phenotype of cells in vivo, but limited recovery from biopsies and restricted lifespan makes experimental manipulation challenging. Therefore, despite cell lines present some limitations, they could be used as an alternative for preliminary experiments.

Original languageEnglish
Pages (from-to)1835-1842
Number of pages8
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume12
Issue number8
DOIs
Publication statusPublished - Aug 2018

Fingerprint

Hyaluronic acid
Platelet-Rich Plasma
Hyaluronic Acid
Platelets
Cell culture
Cells
Plasmas
Cell Line
Osteoarthritis
Vascular Endothelial Growth Factor A
Biopsy
Pathology
Fibroblasts
Gene expression
Chondrocytes
Interleukin-6
Protein Isoforms
Recovery
Cell Culture Techniques
Inflammation

Keywords

  • Immortalized human synoviocytes cell line
  • hyaluronic acid
  • in vitro model
  • osteoarthritis
  • platelet-rich plasma
  • synovial inflammation

Cite this

Cultures of a human synovial cell line to evaluate platelet-rich plasma and hyaluronic acid effects. / Olivotto, Eleonora; Merli, Giulia; Assirelli, Elisa; Cavallo, Carola; Belluzzi, E; Ramonda, R; Favero, M; Filardo, G; Roffi, Alice; Kon, E; Grigolo, Brunella.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 12, No. 8, 08.2018, p. 1835-1842.

Research output: Contribution to journalArticle

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abstract = "Synovial inflammation plays an important role in osteoarthritis (OA) pathogenesis. Different biological compounds have been tested mainly on chondrocytes, to treat early stages of OA. However, since OA has been recently defined as {"}an organ{"} pathology, investigation on synoviocytes is also needed. Therefore the aim of the present study was to validate a human fibroblast-like synoviocytes cell line (K4IM) to test the effects of platelet-rich plasma (PRP) and hyaluronan (HA) on anabolic and catabolic gene expression and on HA secretion from cell cultures. In order to determine the effect of PRP and HA, K4IM cells were maintained in culture with or without TNF-α stimulation. In the presence of PRP, unstimulated K4IM cells presented the same expression of IL1B, IL6, CXCL8, VEGF, TIMP1 and hyaluronic synthase isoform HAS3 as primary human synoviocytes, while HA addition did not change their expression pattern, which was similar to control cells. Stimulated cells expressed significantly higher values of IL1B, CXCL8 and VEGF compared to unstimulated ones. PRP did not show any modification, except for VEGF, while HA addition modulated IL1B expression. PRP did not modulate HA release of both stimulated and unstimulated cells. Our study showed the possibility to use K4IM synoviocytes as an in vitro model to test biological compounds useful for the treatment of early OA. Primary cells reflect the phenotype of cells in vivo, but limited recovery from biopsies and restricted lifespan makes experimental manipulation challenging. Therefore, despite cell lines present some limitations, they could be used as an alternative for preliminary experiments.",
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T1 - Cultures of a human synovial cell line to evaluate platelet-rich plasma and hyaluronic acid effects

AU - Olivotto, Eleonora

AU - Merli, Giulia

AU - Assirelli, Elisa

AU - Cavallo, Carola

AU - Belluzzi, E

AU - Ramonda, R

AU - Favero, M

AU - Filardo, G

AU - Roffi, Alice

AU - Kon, E

AU - Grigolo, Brunella

N1 - This article is protected by copyright. All rights reserved.

PY - 2018/8

Y1 - 2018/8

N2 - Synovial inflammation plays an important role in osteoarthritis (OA) pathogenesis. Different biological compounds have been tested mainly on chondrocytes, to treat early stages of OA. However, since OA has been recently defined as "an organ" pathology, investigation on synoviocytes is also needed. Therefore the aim of the present study was to validate a human fibroblast-like synoviocytes cell line (K4IM) to test the effects of platelet-rich plasma (PRP) and hyaluronan (HA) on anabolic and catabolic gene expression and on HA secretion from cell cultures. In order to determine the effect of PRP and HA, K4IM cells were maintained in culture with or without TNF-α stimulation. In the presence of PRP, unstimulated K4IM cells presented the same expression of IL1B, IL6, CXCL8, VEGF, TIMP1 and hyaluronic synthase isoform HAS3 as primary human synoviocytes, while HA addition did not change their expression pattern, which was similar to control cells. Stimulated cells expressed significantly higher values of IL1B, CXCL8 and VEGF compared to unstimulated ones. PRP did not show any modification, except for VEGF, while HA addition modulated IL1B expression. PRP did not modulate HA release of both stimulated and unstimulated cells. Our study showed the possibility to use K4IM synoviocytes as an in vitro model to test biological compounds useful for the treatment of early OA. Primary cells reflect the phenotype of cells in vivo, but limited recovery from biopsies and restricted lifespan makes experimental manipulation challenging. Therefore, despite cell lines present some limitations, they could be used as an alternative for preliminary experiments.

AB - Synovial inflammation plays an important role in osteoarthritis (OA) pathogenesis. Different biological compounds have been tested mainly on chondrocytes, to treat early stages of OA. However, since OA has been recently defined as "an organ" pathology, investigation on synoviocytes is also needed. Therefore the aim of the present study was to validate a human fibroblast-like synoviocytes cell line (K4IM) to test the effects of platelet-rich plasma (PRP) and hyaluronan (HA) on anabolic and catabolic gene expression and on HA secretion from cell cultures. In order to determine the effect of PRP and HA, K4IM cells were maintained in culture with or without TNF-α stimulation. In the presence of PRP, unstimulated K4IM cells presented the same expression of IL1B, IL6, CXCL8, VEGF, TIMP1 and hyaluronic synthase isoform HAS3 as primary human synoviocytes, while HA addition did not change their expression pattern, which was similar to control cells. Stimulated cells expressed significantly higher values of IL1B, CXCL8 and VEGF compared to unstimulated ones. PRP did not show any modification, except for VEGF, while HA addition modulated IL1B expression. PRP did not modulate HA release of both stimulated and unstimulated cells. Our study showed the possibility to use K4IM synoviocytes as an in vitro model to test biological compounds useful for the treatment of early OA. Primary cells reflect the phenotype of cells in vivo, but limited recovery from biopsies and restricted lifespan makes experimental manipulation challenging. Therefore, despite cell lines present some limitations, they could be used as an alternative for preliminary experiments.

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KW - in vitro model

KW - osteoarthritis

KW - platelet-rich plasma

KW - synovial inflammation

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SN - 1932-6254

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