Cultures of human Schwann cells isolated from fetal nerves

Elio Scarpini, Barbara Q. Kreider, Robert P. Lisak, Giovanni Meola, Maria Elena Velicogna, Pierluigi Baron, Sandro Beretta, Mario Buscaglia, Alonzo H. Ross, Guglielmo Scarlato

Research output: Contribution to journalArticle

Abstract

We describe a technique for the preparation of highly purified populations of Schwann cells (SC) from human fetal nerves. Cultures were prepared by chemical and mechanical dissociation of human fetal sciatic nerves by modification of the method of Kreider et al. developed for newborn rat nerve. A time course analysis of some SC-associated markers at different times in vitro was performed employing immunofluorescence (IF) and immunoperoxidase (IP) to determine the percentage of SC in culture and to evaluate the maintenance of specific SC characteristics. We compared this method with that of Askanss et al. which produces enriched SC cultures by utilizing successive re-explanation of the original nerve explant. After 48 h, approximately 90% of the cells were bipolar and S-100+ and over the next two weeks about 70-80% of cells were SC by cytologic and immunocytologic criteria. At 35 days, 35% were SC, whereas {slanted equal to or less-than}2.5% of 35-day-old multi-explant cultures were SC. The SC obtained by this method displayed the typical morphological and immunological characteristics: they expressed surface laminin and nerve growth factor receptors, whereas fibronectin, which is localized on fibroblast surface, was absent.

Original languageEnglish
Pages (from-to)261-266
Number of pages6
JournalBrain Research
Volume440
Issue number2
DOIs
Publication statusPublished - Feb 9 1988

Keywords

  • Cell culture
  • Fetal nerve
  • Schwann cell

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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  • Cite this

    Scarpini, E., Kreider, B. Q., Lisak, R. P., Meola, G., Velicogna, M. E., Baron, P., Beretta, S., Buscaglia, M., Ross, A. H., & Scarlato, G. (1988). Cultures of human Schwann cells isolated from fetal nerves. Brain Research, 440(2), 261-266. https://doi.org/10.1016/0006-8993(88)90994-8