Curcumin anti-apoptotic action in a model of intestinal epithelial inflammatory damage

Claudia Loganes, Sara Lega, Matteo Bramuzzo, Liza Vecchi Brumatti, Elisa Piscianz, Erica Valencic, Alberto Tommasini, Annalisa Marcuzzi

Research output: Contribution to journalArticle

Abstract

© 2017, MDPI AG. All rights reserved. The purpose of this study is to determine if a preventive treatment with curcumin can protect intestinal epithelial cells from inflammatory damage induced by IFN γ . To achieve this goal we have used a human intestinal epithelial cell line (HT29) treated with IFNIFN γ to undergo apoptotic changes that can reproduce the damage of intestinal epithelia exposed to inflammatory cytokines. In this model, we measured the effect of curcumin (curcuminoid from Curcuma Longa) added as a pre-treatment at different time intervals before stimulation with IFN . Curcumin administration to HT29 culture before the inflammatory stimulus IFNIFN γ reduced the cell apoptosis rate. This effect gradually declined with the reduction of the curcumin pre-incubation time. This anti-apoptotic action by curcumin pre-treatment was paralleled by a reduction of secreted IL7 in the HT29 culture media, while there was no relevant change in the other cytokine levels. Even though curcumin pre-administration did not impact the activation of the NF-ΚB pathway, a slight effect on the phosphorylation of proteins in this inflammatory signaling pathway was observed. In conclusion, curcumin pre-treatment can protect intestinal cells from inflammatory damage. These results can be the basis for studying the preventive role of curcumin in inflammatory bowel diseases.
Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalNutrients
Volume9
Issue number6
DOIs
Publication statusPublished - Jun 6 2017

Keywords

  • Apoptosis
  • Curcumin
  • Cytokine
  • Epithelial cell line
  • Intestinal inflammation

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