Curcumin inhibits activation of Vγ9Vδ2 T cells by phosphoantigens and induces apoptosis involving apoptosis-inducing factor and large scale DNA fragmentation

B. Cipriani, G. Borsellino, H. Knowles, D. Tramonti, F. Cavaliere, G. Bernardi, L. Battistini, C. F. Brosnan

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Curcumin, in addition to its role as a spice, has been used for centuries to treat inflammatory disorders. Although the mechanism of action remains unclear, it has been shown to inhibit the activation of NF-κB and AP-1, transcription factors required for induction of many proinflammatory mediators. Due to its low toxicity it is currently under consideration as a broad anti-inflammatory, anti-tumor cell agent. In this study we investigated whether curcumin inhibited the response of γδ T cells to protease-resistant phosphorylated derivatives found in the cell wall of many pathogens. The results showed that curcumin levels ≥30 μM profoundly inhibited isopentenyl pyrophosphate-induced release of the chemokines macrophage inflammatory protein-1α and -1β and RANTES. Curcumin also blocked isopentenyl pyrophosphate-induced activation of NF-κB and AP-1. Commencing around 16 h, treatment with curcumin lead to the induction of cell death that could not be reversed by APC, IL-15, or IL-2. This cytotoxicity was associated with increased annexin V reactivity, nuclear expression of active caspase-3, cleavage of poly(ADP-ribose) polymerase, translocation of apoptosis-inducing factor to the nucleus, and morphological evidence of nuclear disintegration. However, curcumin led to only large scale DNA chromatolysis, as determined by a combination of TUNEL staining and pulse-field and agarose gel electrophoresis, suggesting a predominantly apoptosis-inducing factor-mediated cell death process. We conclude that γδ T cells activated by these ubiquitous Ags are highly sensitive to curcumin, and that this effect may contribute to the anti-inflammatory properties of this compound.

Original languageEnglish
Pages (from-to)3454-3462
Number of pages9
JournalJournal of Immunology
Volume167
Issue number6
Publication statusPublished - Sep 15 2001

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Apoptosis Inducing Factor
Curcumin
DNA Fragmentation
Apoptosis
T-Lymphocytes
Transcription Factor AP-1
Cell Death
Anti-Inflammatory Agents
Macrophage Inflammatory Proteins
Interleukin-15
Chemokine CCL5
Spices
Poly(ADP-ribose) Polymerases
Agar Gel Electrophoresis
Annexin A5
In Situ Nick-End Labeling
Chemokines
Caspase 3
Cell Wall
Interleukin-2

ASJC Scopus subject areas

  • Immunology

Cite this

Curcumin inhibits activation of Vγ9Vδ2 T cells by phosphoantigens and induces apoptosis involving apoptosis-inducing factor and large scale DNA fragmentation. / Cipriani, B.; Borsellino, G.; Knowles, H.; Tramonti, D.; Cavaliere, F.; Bernardi, G.; Battistini, L.; Brosnan, C. F.

In: Journal of Immunology, Vol. 167, No. 6, 15.09.2001, p. 3454-3462.

Research output: Contribution to journalArticle

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