Current Gel Electrophoresis Approaches to Low-Abundance Protein Marker Discovery

Egisto Boschetti; Giovanni Candiano; Pier Giorgio Righetti

doi:10.1016/B978-0-12-394446-7.00011-X

Current Gel Electrophoresis Approaches to Low-Abundance Protein Marker Discovery

Egisto Boschetti, Giovanni Candiano, Pier Giorgio Righetti

Istituto "Giannina Gaslini"

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Two-dimensional polyacrylamide gel electrophoresis (2-DE) is one of the oldest separation/analytical methods in biochemistry that became progressively the leading technology in proteomics. Over time it has steadily improved in sensitivity, reproducibility, and ease of manipulation. Today it remains irreplaceable in protein investigations. Although it is not the solution to all issues, it is of more interest when associated with complementary technologies capable of enhancing the detection of low-abundance species or expression modifications that intervene at the early stage of a given disease. This chapter describes 2-DE in association with a combinatorial peptide ligand library as an integrated tool to detect species that otherwise would remain undetectable.

Original language	English
Title of host publication	Proteomic and Metabolomic Approaches to Biomarker Discovery
Publisher	Elsevier Inc.
Pages	175-190
Number of pages	16
ISBN (Print)	9780123944467
DOIs	https://doi.org/10.1016/B978-0-12-394446-7.00011-X
Publication status	Published - Jun 2013

Keywords

Biological fluids
Biomarkers
Combinatorial peptide ligand libraries
Sera
Two-dimensional maps

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1016/B978-0-12-394446-7.00011-X

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abstract = "Two-dimensional polyacrylamide gel electrophoresis (2-DE) is one of the oldest separation/analytical methods in biochemistry that became progressively the leading technology in proteomics. Over time it has steadily improved in sensitivity, reproducibility, and ease of manipulation. Today it remains irreplaceable in protein investigations. Although it is not the solution to all issues, it is of more interest when associated with complementary technologies capable of enhancing the detection of low-abundance species or expression modifications that intervene at the early stage of a given disease. This chapter describes 2-DE in association with a combinatorial peptide ligand library as an integrated tool to detect species that otherwise would remain undetectable.",

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AU - Righetti, Pier Giorgio

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KW - Two-dimensional maps

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ER -

Current Gel Electrophoresis Approaches to Low-Abundance Protein Marker Discovery

Abstract

Keywords

ASJC Scopus subject areas

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