Abstract
With the advent of proteomic techniques the number of known post-translational modifications (PTMs) affecting red cell membrane proteins is rapidly growing but the understanding of their role under physiological and pathological conditions is incompletely established. The wide range of hereditary diseases affecting different red cell membrane functions and the membrane modifications induced by malaria parasite intracellular growth represent a unique opportunity to study PTMs in response to variable cellular stresses. In the present review, some of the major areas of interest in red cell membrane research have been considered as modifications of erythrocyte deformability and maintenance of the surface area, membrane transport alterations, and removal of diseased and senescent red cells. In all mentioned research areas the functional roles of PTMs are prevalently restricted to the phosphorylative changes of the more abundant membrane proteins. The insufficient information about the PTMs occurring in a large majority of the red membrane proteins and the general lack of mass spectrometry data evidence the need of new comprehensive, proteomic approaches to improve the understanding of the red cell membrane physiology.
| Original language | English |
|---|---|
| Pages (from-to) | 445-455 |
| Number of pages | 11 |
| Journal | Journal of Proteomics |
| Volume | 73 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jan 3 2010 |
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Keywords
- Erythrocyte removal
- Hemolytic disease
- Phosphorylative changes
- Src family kinases
ASJC Scopus subject areas
- Biochemistry
- Biophysics
Cite this
Current knowledge about the functional roles of phosphorylative changes of membrane proteins in normal and diseased red cells. / Pantaleo, Antonella; De Franceschi, Lucia; Ferru, Emanuela; Vono, Rosa; Turrini, Franco.
In: Journal of Proteomics, Vol. 73, No. 3, 03.01.2010, p. 445-455.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Current knowledge about the functional roles of phosphorylative changes of membrane proteins in normal and diseased red cells
AU - Pantaleo, Antonella
AU - De Franceschi, Lucia
AU - Ferru, Emanuela
AU - Vono, Rosa
AU - Turrini, Franco
PY - 2010/1/3
Y1 - 2010/1/3
N2 - With the advent of proteomic techniques the number of known post-translational modifications (PTMs) affecting red cell membrane proteins is rapidly growing but the understanding of their role under physiological and pathological conditions is incompletely established. The wide range of hereditary diseases affecting different red cell membrane functions and the membrane modifications induced by malaria parasite intracellular growth represent a unique opportunity to study PTMs in response to variable cellular stresses. In the present review, some of the major areas of interest in red cell membrane research have been considered as modifications of erythrocyte deformability and maintenance of the surface area, membrane transport alterations, and removal of diseased and senescent red cells. In all mentioned research areas the functional roles of PTMs are prevalently restricted to the phosphorylative changes of the more abundant membrane proteins. The insufficient information about the PTMs occurring in a large majority of the red membrane proteins and the general lack of mass spectrometry data evidence the need of new comprehensive, proteomic approaches to improve the understanding of the red cell membrane physiology.
AB - With the advent of proteomic techniques the number of known post-translational modifications (PTMs) affecting red cell membrane proteins is rapidly growing but the understanding of their role under physiological and pathological conditions is incompletely established. The wide range of hereditary diseases affecting different red cell membrane functions and the membrane modifications induced by malaria parasite intracellular growth represent a unique opportunity to study PTMs in response to variable cellular stresses. In the present review, some of the major areas of interest in red cell membrane research have been considered as modifications of erythrocyte deformability and maintenance of the surface area, membrane transport alterations, and removal of diseased and senescent red cells. In all mentioned research areas the functional roles of PTMs are prevalently restricted to the phosphorylative changes of the more abundant membrane proteins. The insufficient information about the PTMs occurring in a large majority of the red membrane proteins and the general lack of mass spectrometry data evidence the need of new comprehensive, proteomic approaches to improve the understanding of the red cell membrane physiology.
KW - Erythrocyte removal
KW - Hemolytic disease
KW - Phosphorylative changes
KW - Src family kinases
UR - http://www.scopus.com/inward/record.url?scp=72149101507&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=72149101507&partnerID=8YFLogxK
U2 - 10.1016/j.jprot.2009.08.011
DO - 10.1016/j.jprot.2009.08.011
M3 - Article
C2 - 19758581
AN - SCOPUS:72149101507
VL - 73
SP - 445
EP - 455
JO - Journal of Proteomics
JF - Journal of Proteomics
SN - 1874-3919
IS - 3
ER -