Invariant Vα14 (Vα14i) NKT cells are a murine CD1d-dependent regulatory T cell subset characterized by a Vα14-Jα18 rearrangement and expression of mostly Vβ8.2 and Vβ7. Whereas the TCR Vβ domain influences the binding avidity of the Vα14i TCRfor CD1d-α- galactosylceramide complexes, with Vβ8.2 conferring higher avidity binding than Vβ7, a possible impact of the TCR Vβ domain on Vα14i NKT cell selection by endogenous ligands has not been studied. In this study, we show that thymic selection of Vβ7+, but not Vβ8.2 +, Vα14i NKT cells is favored in situations where endogenous ligand concentration or TCRα-chain avidity are suboptimal. Furthermore, thymic Vβ7+ Vα14i NKT cells were preferentially selected in vitro in response to CD1d-dependent presentation of endogenous ligands or exogenously added self ligand isoglobotrihexosylceramide. Collectively, our data demonstrate that the TCR Vβ domain influences the selection of Vα14i NKT cells by endogenous ligands, presumably because Vβ7 confers higher avidity binding.
|Number of pages||5|
|Journal||Journal of Immunology|
|Publication status||Published - Feb 15 2006|
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