CX3CL1 protects neurons against excitotoxicity enhancing GLT-1 activity on astrocytes

Myriam Catalano, Clotilde Lauro, Raffaela Cipriani, Giuseppina Chece, Andrea Ponzetta, Silvia Di Angelantonio, Davide Ragozzino, Cristina Limatola

Research output: Contribution to journalArticle

Abstract

In this paper we show for the first time that: i) astrocytes are required for the neuroprotective activity of CX3CL1 against excitotoxicity; ii) inhibition of the glutamate transporter 1 (GLT-1) prejudices CX3CL1-mediated neuroprotection; iii) CX3CL1 increases GLT-1 activity on astrocytes. The modulation of GLT-1 activity induced by CX3CL1 on astrocytes requires the presence and the activity of A1 adenosine receptor (A1R), being blocked by the specific antagonist DPCPX and absent in A1R-/- astrocytes. These data introduce the astrocytes as active players in CX3CL1-mediated signaling between microglia and neurons, identifying GLT-1 as a key mediator of the neuroprotective activity of CX3CL1.

Original languageEnglish
Pages (from-to)75-82
Number of pages8
JournalJournal of Neuroimmunology
Volume263
Issue number1-2
DOIs
Publication statusPublished - Oct 15 2013

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Amino Acid Transport System X-AG
Astrocytes
Neurons
Adenosine A1 Receptors
Microglia

Keywords

  • A1R
  • Astrocytes
  • CX3CL1
  • GLT-1
  • Microglia
  • Neuroprotection

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

Cite this

CX3CL1 protects neurons against excitotoxicity enhancing GLT-1 activity on astrocytes. / Catalano, Myriam; Lauro, Clotilde; Cipriani, Raffaela; Chece, Giuseppina; Ponzetta, Andrea; Di Angelantonio, Silvia; Ragozzino, Davide; Limatola, Cristina.

In: Journal of Neuroimmunology, Vol. 263, No. 1-2, 15.10.2013, p. 75-82.

Research output: Contribution to journalArticle

Catalano, M, Lauro, C, Cipriani, R, Chece, G, Ponzetta, A, Di Angelantonio, S, Ragozzino, D & Limatola, C 2013, 'CX3CL1 protects neurons against excitotoxicity enhancing GLT-1 activity on astrocytes', Journal of Neuroimmunology, vol. 263, no. 1-2, pp. 75-82. https://doi.org/10.1016/j.jneuroim.2013.07.020
Catalano M, Lauro C, Cipriani R, Chece G, Ponzetta A, Di Angelantonio S et al. CX3CL1 protects neurons against excitotoxicity enhancing GLT-1 activity on astrocytes. Journal of Neuroimmunology. 2013 Oct 15;263(1-2):75-82. https://doi.org/10.1016/j.jneuroim.2013.07.020
Catalano, Myriam ; Lauro, Clotilde ; Cipriani, Raffaela ; Chece, Giuseppina ; Ponzetta, Andrea ; Di Angelantonio, Silvia ; Ragozzino, Davide ; Limatola, Cristina. / CX3CL1 protects neurons against excitotoxicity enhancing GLT-1 activity on astrocytes. In: Journal of Neuroimmunology. 2013 ; Vol. 263, No. 1-2. pp. 75-82.
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