CXC chemokines interleukin-8 (IL-8) and growth-related gene product α (GROα) modulate Purkinje neuron activity in mouse cerebellum

Aldo Giovannelli, Cristina Limatola, Davide Ragozzino, Anna Maria Mileo, Alessandro Ruggieri, Maria Teresa Ciotti, Delio Mercanti, Angela Santoni, Fabrizio Eusebi

Research output: Contribution to journalArticlepeer-review

Abstract

We give here evidence that Purkinje neurons (PNs) of mouse cerebellar slices studied with patch clamp technique combined with laser confocal microscopy, respond to human IL-8 and GROα by (i) a cytosolic Ca2+ transient compatible with inositol (1,4,5) trisphosphate (InsP3) formation; (ii) an enhancement of the neurotransmitter release; and (iii) an impairment of the long-term depression of synaptic strength (LTD). It was also found the expression of IL-8 receptor type 2 in PN and granule cells by immunofluorescence, immunoblotting and RT-PCR analysis. Considered together these findings suggest that IL-8 and GROα may play a neuromodulatory role on mouse cerebellum.

Original languageEnglish
Pages (from-to)122-132
Number of pages11
JournalJournal of Neuroimmunology
Volume92
Issue number1-2
DOIs
Publication statusPublished - Dec 1998

Keywords

  • Ca
  • Chemokines
  • CXCR
  • Granule cells
  • Inositol (1,4,5) trisphosphate
  • Long-term depression

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

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