CXC chemokines interleukin-8 (IL-8) and growth-related gene product α (GROα) modulate Purkinje neuron activity in mouse cerebellum

Aldo Giovannelli; Cristina Limatola; Davide Ragozzino; Anna Maria Mileo; Alessandro Ruggieri; Maria Teresa Ciotti; Delio Mercanti; Angela Santoni; Fabrizio Eusebi

doi:10.1016/S0165-5728(98)00192-1

CXC chemokines interleukin-8 (IL-8) and growth-related gene product α (GROα) modulate Purkinje neuron activity in mouse cerebellum

Aldo Giovannelli, Cristina Limatola, Davide Ragozzino, Anna Maria Mileo, Alessandro Ruggieri, Maria Teresa Ciotti, Delio Mercanti, Angela Santoni, Fabrizio Eusebi

Research output: Contribution to journal › Article › peer-review

Abstract

We give here evidence that Purkinje neurons (PNs) of mouse cerebellar slices studied with patch clamp technique combined with laser confocal microscopy, respond to human IL-8 and GROα by (i) a cytosolic Ca²⁺ transient compatible with inositol (1,4,5) trisphosphate (InsP₃) formation; (ii) an enhancement of the neurotransmitter release; and (iii) an impairment of the long-term depression of synaptic strength (LTD). It was also found the expression of IL-8 receptor type 2 in PN and granule cells by immunofluorescence, immunoblotting and RT-PCR analysis. Considered together these findings suggest that IL-8 and GROα may play a neuromodulatory role on mouse cerebellum.

Original language	English
Pages (from-to)	122-132
Number of pages	11
Journal	Journal of Neuroimmunology
Volume	92
Issue number	1-2
DOIs	https://doi.org/10.1016/S0165-5728(98)00192-1
Publication status	Published - Dec 1998

Keywords

Ca
Chemokines
CXCR
Granule cells
Inositol (1,4,5) trisphosphate
Long-term depression

ASJC Scopus subject areas

Immunology
Clinical Neurology
Immunology and Allergy
Neurology

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CXC chemokines interleukin-8 (IL-8) and growth-related gene product α (GROα) modulate Purkinje neuron activity in mouse cerebellum. / Giovannelli, Aldo; Limatola, Cristina; Ragozzino, Davide; Mileo, Anna Maria; Ruggieri, Alessandro; Ciotti, Maria Teresa; Mercanti, Delio; Santoni, Angela; Eusebi, Fabrizio.

In: Journal of Neuroimmunology, Vol. 92, No. 1-2, 12.1998, p. 122-132.

Research output: Contribution to journal › Article › peer-review

Giovannelli, Aldo ; Limatola, Cristina ; Ragozzino, Davide ; Mileo, Anna Maria ; Ruggieri, Alessandro ; Ciotti, Maria Teresa ; Mercanti, Delio ; Santoni, Angela ; Eusebi, Fabrizio. / CXC chemokines interleukin-8 (IL-8) and growth-related gene product α (GROα) modulate Purkinje neuron activity in mouse cerebellum. In: Journal of Neuroimmunology. 1998 ; Vol. 92, No. 1-2. pp. 122-132.

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title = "CXC chemokines interleukin-8 (IL-8) and growth-related gene product α (GROα) modulate Purkinje neuron activity in mouse cerebellum",

abstract = "We give here evidence that Purkinje neurons (PNs) of mouse cerebellar slices studied with patch clamp technique combined with laser confocal microscopy, respond to human IL-8 and GROα by (i) a cytosolic Ca2+ transient compatible with inositol (1,4,5) trisphosphate (InsP3) formation; (ii) an enhancement of the neurotransmitter release; and (iii) an impairment of the long-term depression of synaptic strength (LTD). It was also found the expression of IL-8 receptor type 2 in PN and granule cells by immunofluorescence, immunoblotting and RT-PCR analysis. Considered together these findings suggest that IL-8 and GROα may play a neuromodulatory role on mouse cerebellum.",

keywords = "Ca, Chemokines, CXCR, Granule cells, Inositol (1,4,5) trisphosphate, Long-term depression",

author = "Aldo Giovannelli and Cristina Limatola and Davide Ragozzino and Mileo, {Anna Maria} and Alessandro Ruggieri and Ciotti, {Maria Teresa} and Delio Mercanti and Angela Santoni and Fabrizio Eusebi",

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AU - Giovannelli, Aldo

AU - Limatola, Cristina

AU - Ragozzino, Davide

AU - Mileo, Anna Maria

AU - Ruggieri, Alessandro

AU - Ciotti, Maria Teresa

AU - Mercanti, Delio

AU - Santoni, Angela

AU - Eusebi, Fabrizio

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N2 - We give here evidence that Purkinje neurons (PNs) of mouse cerebellar slices studied with patch clamp technique combined with laser confocal microscopy, respond to human IL-8 and GROα by (i) a cytosolic Ca2+ transient compatible with inositol (1,4,5) trisphosphate (InsP3) formation; (ii) an enhancement of the neurotransmitter release; and (iii) an impairment of the long-term depression of synaptic strength (LTD). It was also found the expression of IL-8 receptor type 2 in PN and granule cells by immunofluorescence, immunoblotting and RT-PCR analysis. Considered together these findings suggest that IL-8 and GROα may play a neuromodulatory role on mouse cerebellum.

AB - We give here evidence that Purkinje neurons (PNs) of mouse cerebellar slices studied with patch clamp technique combined with laser confocal microscopy, respond to human IL-8 and GROα by (i) a cytosolic Ca2+ transient compatible with inositol (1,4,5) trisphosphate (InsP3) formation; (ii) an enhancement of the neurotransmitter release; and (iii) an impairment of the long-term depression of synaptic strength (LTD). It was also found the expression of IL-8 receptor type 2 in PN and granule cells by immunofluorescence, immunoblotting and RT-PCR analysis. Considered together these findings suggest that IL-8 and GROα may play a neuromodulatory role on mouse cerebellum.

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KW - Chemokines

KW - CXCR

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KW - Inositol (1,4,5) trisphosphate

KW - Long-term depression

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CXC chemokines interleukin-8 (IL-8) and growth-related gene product α (GROα) modulate Purkinje neuron activity in mouse cerebellum

Abstract

Keywords

ASJC Scopus subject areas

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