TY - JOUR
T1 - CXC chemokines interleukin-8 (IL-8) and growth-related gene product α (GROα) modulate Purkinje neuron activity in mouse cerebellum
AU - Giovannelli, Aldo
AU - Limatola, Cristina
AU - Ragozzino, Davide
AU - Mileo, Anna Maria
AU - Ruggieri, Alessandro
AU - Ciotti, Maria Teresa
AU - Mercanti, Delio
AU - Santoni, Angela
AU - Eusebi, Fabrizio
PY - 1998/12
Y1 - 1998/12
N2 - We give here evidence that Purkinje neurons (PNs) of mouse cerebellar slices studied with patch clamp technique combined with laser confocal microscopy, respond to human IL-8 and GROα by (i) a cytosolic Ca2+ transient compatible with inositol (1,4,5) trisphosphate (InsP3) formation; (ii) an enhancement of the neurotransmitter release; and (iii) an impairment of the long-term depression of synaptic strength (LTD). It was also found the expression of IL-8 receptor type 2 in PN and granule cells by immunofluorescence, immunoblotting and RT-PCR analysis. Considered together these findings suggest that IL-8 and GROα may play a neuromodulatory role on mouse cerebellum.
AB - We give here evidence that Purkinje neurons (PNs) of mouse cerebellar slices studied with patch clamp technique combined with laser confocal microscopy, respond to human IL-8 and GROα by (i) a cytosolic Ca2+ transient compatible with inositol (1,4,5) trisphosphate (InsP3) formation; (ii) an enhancement of the neurotransmitter release; and (iii) an impairment of the long-term depression of synaptic strength (LTD). It was also found the expression of IL-8 receptor type 2 in PN and granule cells by immunofluorescence, immunoblotting and RT-PCR analysis. Considered together these findings suggest that IL-8 and GROα may play a neuromodulatory role on mouse cerebellum.
KW - Ca
KW - Chemokines
KW - CXCR
KW - Granule cells
KW - Inositol (1,4,5) trisphosphate
KW - Long-term depression
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U2 - 10.1016/S0165-5728(98)00192-1
DO - 10.1016/S0165-5728(98)00192-1
M3 - Article
C2 - 9916887
AN - SCOPUS:0032414498
VL - 92
SP - 122
EP - 132
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
SN - 0165-5728
IS - 1-2
ER -