Abstract
Recent evidence indicates that cancer cells express chemokine (CK) receptors and that their signaling is crucial for tumor proliferation, migration, and angiogenesis. The profiles of expression of CXC CK receptors (CXCR1-5) and their main ligands (growth-related oncogene, GRO1-2-3/CXCL1-2-3; interleukin 8, IL-8/CXCL8; monokine-induced γ-interferon MIG/CXCL9; γ-interferon-inducible-protein-10, IP-10/CXCL10; stromal cell-derived factor-1, SDF1/CXCL12; B-cell activating CK-1, BCA-1/CXCL13) were analyzed by reverse transcription polymerase chain reaction (RT-PCR) in surgical samples of human meningiomas. All the five receptors displayed high percentages of positive cases: 92% CXCR1, 89% CXCR2, 83% CXCR3, 78% CXCR4, and 94% CXCR5. Conversely, their ligands showed a lower pattern of expression: 40% IL-8, 42% GRO1-3, 42% IP-10, 28% MIG, 53% SDF1, and 3% BCA-1. SDF1/CXCR4 interaction plays a pivotal role in cancer proliferation. Thus, the signaling mechanisms activated by the exclusive binding between SDF1 and CXCR4 was investigated in 12 primary cultures from meningioma tissues. CXCR4 was functionally coupled as demonstrated by the significant increase of DNA synthesis in meningioma cells in response to SDF1, measured by [3H]-thymidine uptake. In three primary cultures, the SDF1-dependent mitogenic activity was associated with a marked phosphorylation of extracellular signal-regulated kinase (ERK1/2) as evaluated by Western blots. PD98059 (a MEK inhibitor) significantly reduced ERK1/2 activation, thus linking the SDF1/CXCR4 pathway to meningioma cell proliferation via ERK1/2 signal transduction. We demonstrate, for the first time in human meningiomas, the simultaneous expression of CXCR1-5 and their CKs and the mitogenic activity of SDF1/CXCR4, suggesting a pivotal role of these receptor-ligand pairs in meningeal tumors.
| Original language | English |
|---|---|
| Title of host publication | Annals of the New York Academy of Sciences |
| Pages | 332-343 |
| Number of pages | 12 |
| Volume | 1090 |
| DOIs | |
| Publication status | Published - Dec 2006 |
Publication series
| Name | Annals of the New York Academy of Sciences |
|---|---|
| Volume | 1090 |
| ISSN (Print) | 00778923 |
| ISSN (Electronic) | 17496632 |
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Keywords
- Cell proliferation
- Chemokine
- CXCR4
- ERK1/2
- Meningioma
- Primary culture
- Receptor
- SDF1
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
CXC receptor and chemokine expression in human meningioma : SDF1/CXCR4 signaling activates ERK1/2 and stimulates meningioma cell proliferation. / Barbieri, Federica; Bajetto, Adriana; Porcile, Carola; Pattarozzi, Alessandra; Massa, Alessandro; Lunardi, Gianluigi; Zona, Gianluigi; Dorcaratto, Alessandra; Ravetti, Jean Louis; Spaziante, Renato; Schettini, Gennaro; Florio, Tullio.
Annals of the New York Academy of Sciences. Vol. 1090 2006. p. 332-343 (Annals of the New York Academy of Sciences; Vol. 1090).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - CXC receptor and chemokine expression in human meningioma
T2 - SDF1/CXCR4 signaling activates ERK1/2 and stimulates meningioma cell proliferation
AU - Barbieri, Federica
AU - Bajetto, Adriana
AU - Porcile, Carola
AU - Pattarozzi, Alessandra
AU - Massa, Alessandro
AU - Lunardi, Gianluigi
AU - Zona, Gianluigi
AU - Dorcaratto, Alessandra
AU - Ravetti, Jean Louis
AU - Spaziante, Renato
AU - Schettini, Gennaro
AU - Florio, Tullio
PY - 2006/12
Y1 - 2006/12
N2 - Recent evidence indicates that cancer cells express chemokine (CK) receptors and that their signaling is crucial for tumor proliferation, migration, and angiogenesis. The profiles of expression of CXC CK receptors (CXCR1-5) and their main ligands (growth-related oncogene, GRO1-2-3/CXCL1-2-3; interleukin 8, IL-8/CXCL8; monokine-induced γ-interferon MIG/CXCL9; γ-interferon-inducible-protein-10, IP-10/CXCL10; stromal cell-derived factor-1, SDF1/CXCL12; B-cell activating CK-1, BCA-1/CXCL13) were analyzed by reverse transcription polymerase chain reaction (RT-PCR) in surgical samples of human meningiomas. All the five receptors displayed high percentages of positive cases: 92% CXCR1, 89% CXCR2, 83% CXCR3, 78% CXCR4, and 94% CXCR5. Conversely, their ligands showed a lower pattern of expression: 40% IL-8, 42% GRO1-3, 42% IP-10, 28% MIG, 53% SDF1, and 3% BCA-1. SDF1/CXCR4 interaction plays a pivotal role in cancer proliferation. Thus, the signaling mechanisms activated by the exclusive binding between SDF1 and CXCR4 was investigated in 12 primary cultures from meningioma tissues. CXCR4 was functionally coupled as demonstrated by the significant increase of DNA synthesis in meningioma cells in response to SDF1, measured by [3H]-thymidine uptake. In three primary cultures, the SDF1-dependent mitogenic activity was associated with a marked phosphorylation of extracellular signal-regulated kinase (ERK1/2) as evaluated by Western blots. PD98059 (a MEK inhibitor) significantly reduced ERK1/2 activation, thus linking the SDF1/CXCR4 pathway to meningioma cell proliferation via ERK1/2 signal transduction. We demonstrate, for the first time in human meningiomas, the simultaneous expression of CXCR1-5 and their CKs and the mitogenic activity of SDF1/CXCR4, suggesting a pivotal role of these receptor-ligand pairs in meningeal tumors.
AB - Recent evidence indicates that cancer cells express chemokine (CK) receptors and that their signaling is crucial for tumor proliferation, migration, and angiogenesis. The profiles of expression of CXC CK receptors (CXCR1-5) and their main ligands (growth-related oncogene, GRO1-2-3/CXCL1-2-3; interleukin 8, IL-8/CXCL8; monokine-induced γ-interferon MIG/CXCL9; γ-interferon-inducible-protein-10, IP-10/CXCL10; stromal cell-derived factor-1, SDF1/CXCL12; B-cell activating CK-1, BCA-1/CXCL13) were analyzed by reverse transcription polymerase chain reaction (RT-PCR) in surgical samples of human meningiomas. All the five receptors displayed high percentages of positive cases: 92% CXCR1, 89% CXCR2, 83% CXCR3, 78% CXCR4, and 94% CXCR5. Conversely, their ligands showed a lower pattern of expression: 40% IL-8, 42% GRO1-3, 42% IP-10, 28% MIG, 53% SDF1, and 3% BCA-1. SDF1/CXCR4 interaction plays a pivotal role in cancer proliferation. Thus, the signaling mechanisms activated by the exclusive binding between SDF1 and CXCR4 was investigated in 12 primary cultures from meningioma tissues. CXCR4 was functionally coupled as demonstrated by the significant increase of DNA synthesis in meningioma cells in response to SDF1, measured by [3H]-thymidine uptake. In three primary cultures, the SDF1-dependent mitogenic activity was associated with a marked phosphorylation of extracellular signal-regulated kinase (ERK1/2) as evaluated by Western blots. PD98059 (a MEK inhibitor) significantly reduced ERK1/2 activation, thus linking the SDF1/CXCR4 pathway to meningioma cell proliferation via ERK1/2 signal transduction. We demonstrate, for the first time in human meningiomas, the simultaneous expression of CXCR1-5 and their CKs and the mitogenic activity of SDF1/CXCR4, suggesting a pivotal role of these receptor-ligand pairs in meningeal tumors.
KW - Cell proliferation
KW - Chemokine
KW - CXCR4
KW - ERK1/2
KW - Meningioma
KW - Primary culture
KW - Receptor
KW - SDF1
UR - http://www.scopus.com/inward/record.url?scp=34247641688&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34247641688&partnerID=8YFLogxK
U2 - 10.1196/annals.1378.037
DO - 10.1196/annals.1378.037
M3 - Conference contribution
C2 - 17384278
AN - SCOPUS:34247641688
SN - 1573316458
SN - 9781573316453
VL - 1090
T3 - Annals of the New York Academy of Sciences
SP - 332
EP - 343
BT - Annals of the New York Academy of Sciences
ER -