CXCL12/SDF1 expression by breast cancers is an independent prognostic marker of disease-free and overall survival

Valentina Mirisola, Ambra Zuccarino, Beatrice E. Bachmeier, Maria Pia Sormani, Judith Falter, Andreas Nerlich, Ulrich Pfeffer

Research output: Contribution to journalArticle

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Abstract

The cytokine C-X-C motif chemokine 12 (CXCL12) is synthesised by metastasis target tissues and has been shown to attract tumour cells that express the receptor, C-X-C chemokine receptor type 4 (CXCR4). However, epigenetic silencing of CXCL12 has recently been reported to increase the metastatic potential of breast cancer cells and the reintroduction of the cytokine gene into MDA-MB-231 breast carcinoma cells decreases the number of metastases formed in vivo. We therefore wished to know whether CXCL12 expression correlates with relapse-free and overall survival in human breast cancer patients. The expression of C-X-C motif chemokine 12 (CXCL12) and C-X-C chemokine receptor type 4 (CXCR4) was analysed in 100 archival breast cancer samples by immunohistochemistry and in two breast cancer microarray datasets of 408 cases. Data were analysed by univariate and multivariate COX regression analyses. CXCL12 and CXCR4 are expressed by epithelial tumour cells and by stromal and endothelial cells. Microarray gene expression analysis and immunohistochemistry revealed that expression of CXCL12 but not of CXCR4 significantly correlates with disease-free and overall survival in oestrogen receptor-positive and -negative cancers. The expression of the oestrogen receptor α and that of CXCL12 do not correlate. CXCL12 is a strong, independent prognostic marker. We propose that saturation of the receptor through autocrine CXCL12 production reduces chemotaxis towards CXCL12-releasing metastasis target tissues.

Original languageEnglish
Pages (from-to)2579-2587
Number of pages9
JournalEuropean Journal of Cancer
Volume45
Issue number14
DOIs
Publication statusPublished - Sep 2009

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CXC Chemokines
Disease-Free Survival
Chemokine Receptors
Breast Neoplasms
Chemokine CXCL12
Neoplasm Metastasis
Estrogen Receptors
Immunohistochemistry
Cytokines
Neoplasms
Chemotaxis
Stromal Cells
Epigenomics
Endothelial Cells
Cell Count
Epithelial Cells
Regression Analysis
Gene Expression
Recurrence
Survival

Keywords

  • Breast cancer
  • Chemokine
  • Metastasis
  • Prognostic marker

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

CXCL12/SDF1 expression by breast cancers is an independent prognostic marker of disease-free and overall survival. / Mirisola, Valentina; Zuccarino, Ambra; Bachmeier, Beatrice E.; Sormani, Maria Pia; Falter, Judith; Nerlich, Andreas; Pfeffer, Ulrich.

In: European Journal of Cancer, Vol. 45, No. 14, 09.2009, p. 2579-2587.

Research output: Contribution to journalArticle

Mirisola, Valentina ; Zuccarino, Ambra ; Bachmeier, Beatrice E. ; Sormani, Maria Pia ; Falter, Judith ; Nerlich, Andreas ; Pfeffer, Ulrich. / CXCL12/SDF1 expression by breast cancers is an independent prognostic marker of disease-free and overall survival. In: European Journal of Cancer. 2009 ; Vol. 45, No. 14. pp. 2579-2587.
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