CXCL13, CCL21, and CXCL12 expression in salivary glands of patients with Sjögren's syndrome and MALT lymphoma: Association with reactive and malignant areas of lymphoid organization

Francesca Barone, Michele Bombardieri, Manuela Maria Rosado, Peter Roger Morgan, Stephen J. Challacombe, Salvatore De Vita, Rita Carsetti, Jo Spencer, Guido Valesini, Costantino Pitzalis

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

The chemokines (CKs) CXCL13, CCL21, and CXCL12 are known to play differential roles in the organization of the lymphoid tissues and the development of lymphoid malignancies. We investigated the expression of these CKs and their receptors in the salivary glands of Sjogren's syndrome patients with lymphoepithelial lesions (lymphoepithelial sialadenitis or LESA) and in MALT lymphoma to understand their involvement in salivary gland lymphomagenesis. We demonstrate that within salivary glands with LESA and MALT lymphoma the lymphoid CKs CXCL13 and CCL21 are selectively associated with areas of reactive lymphoid proliferation, whereas no significant expression of these molecules was detected in the malignant lymphoid aggregate. Conversely, CXCL12 was observed predominantly in infiltrated ducts and malignant B cells. Accordingly, CXCL13 and CCL21 transcript levels were significantly increased in LESA samples while CXCL12 levels were increased in MALT lymphoma and isolated tumor cells. Low levels of CK receptors were detected on lymphoma-extracted lymphocytes, suggesting down-regulation in the abundance of ligands. Our findings suggest that in salivary gland MALT lymphoma the lymphoid CKs CXCL13 and CCL21 are directly implicated in the organization of ectopic reactive lymphoid tissue, whereas CXCL12 is associated with the infiltrated epithelium and malignant B cell component and is possibly involved in the regulation of malignant B cell survival in the regulation of malignant B cell survival.

Original languageEnglish
Pages (from-to)5130-5140
Number of pages11
JournalJournal of Immunology
Volume180
Issue number7
Publication statusPublished - Apr 1 2008

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Chemokine CXCL13
Marginal Zone B-Cell Lymphoma
Chemokine CCL21
Salivary Glands
B-Lymphocytes
Chemokine Receptors
Cell Survival
Sialadenitis
Chemokine CXCL12
Sjogren's Syndrome
Lymphoid Tissue
Cellular Structures
Lymphoma
Neoplasms
Down-Regulation
Epithelium
Lymphocytes
Ligands

ASJC Scopus subject areas

  • Immunology

Cite this

Barone, F., Bombardieri, M., Rosado, M. M., Morgan, P. R., Challacombe, S. J., De Vita, S., ... Pitzalis, C. (2008). CXCL13, CCL21, and CXCL12 expression in salivary glands of patients with Sjögren's syndrome and MALT lymphoma: Association with reactive and malignant areas of lymphoid organization. Journal of Immunology, 180(7), 5130-5140.

CXCL13, CCL21, and CXCL12 expression in salivary glands of patients with Sjögren's syndrome and MALT lymphoma : Association with reactive and malignant areas of lymphoid organization. / Barone, Francesca; Bombardieri, Michele; Rosado, Manuela Maria; Morgan, Peter Roger; Challacombe, Stephen J.; De Vita, Salvatore; Carsetti, Rita; Spencer, Jo; Valesini, Guido; Pitzalis, Costantino.

In: Journal of Immunology, Vol. 180, No. 7, 01.04.2008, p. 5130-5140.

Research output: Contribution to journalArticle

Barone, F, Bombardieri, M, Rosado, MM, Morgan, PR, Challacombe, SJ, De Vita, S, Carsetti, R, Spencer, J, Valesini, G & Pitzalis, C 2008, 'CXCL13, CCL21, and CXCL12 expression in salivary glands of patients with Sjögren's syndrome and MALT lymphoma: Association with reactive and malignant areas of lymphoid organization', Journal of Immunology, vol. 180, no. 7, pp. 5130-5140.
Barone, Francesca ; Bombardieri, Michele ; Rosado, Manuela Maria ; Morgan, Peter Roger ; Challacombe, Stephen J. ; De Vita, Salvatore ; Carsetti, Rita ; Spencer, Jo ; Valesini, Guido ; Pitzalis, Costantino. / CXCL13, CCL21, and CXCL12 expression in salivary glands of patients with Sjögren's syndrome and MALT lymphoma : Association with reactive and malignant areas of lymphoid organization. In: Journal of Immunology. 2008 ; Vol. 180, No. 7. pp. 5130-5140.
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AU - Rosado, Manuela Maria

AU - Morgan, Peter Roger

AU - Challacombe, Stephen J.

AU - De Vita, Salvatore

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