CXCL1/Macrophage Inflammatory Protein-2-Induced Angiogenesis In Vivo Is Mediated by Neutrophil-Derived Vascular Endothelial Growth Factor-A

Patrizia Scapini, Monica Morini, Cristina Tecchio, Simona Minghelli, Emma Di Carlo, Elena Tanghetti, Adriana Albini, Clifford Lowell, Giorgio Berton, Douglas M. Noonan, Marco A. Cassatella

Research output: Contribution to journalArticle

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Abstract

The angiogenic activity of CXC-ELR+ chemokines, including CXCL8/IL-8, CXCL1/macrophage inflammatory protein-2 (MIP-2), and CXCL1/growth-related oncogene-α in the Matrigel sponge angiogenesis assay in vivo, is strictly neutrophil dependent, as neutrophil depletion of the animals completely abrogates the angiogenic response. In this study, we demonstrate that mice deficient in the src family kinases, Hck and Fgr (hck -/-fgr-/-), are unable to develop an angiogenic response to CXCL1/MIP-2, although they respond normally to vascular endothelial growth factor-A (VEGF-A). Histological examination of the CXCL1/MIP-2-containing Matrigel implants isolated from wild-type or hck-/-fgr-/- mice showed the presence of an extensive neutrophil infiltrate, excluding a defective neutrophil recruitment into the Matrigel sponges. Accordingly, neutrophils from hck-/-fgr-/- mice normally migrated and released gelatinase B in response to CXCL1/MIP-2 in vitro, similarly to wild-type neutrophils. However, unlike wild-type neutrophils, those from hck-/-fgr-/- mice were completely unable to release VEGF-A upon stimulation with CXCL1/MIP-2. Furthermore, neutralizing anti-VEGF-A Abs abrogated the angiogenic response to CXCL1/MIP-2 in wild-type mice and CXCL1/MIP-2 induced angiogenesis in the chick embryo chorioallantoic membrane assay, indicating that neutrophil-derived VEGF-A is a major mediator of CXCL1/MIP-2-induced angiogenesis. Finally, in vitro kinase assays confirmed that CXCL1/MIP-2 activates Hck and Fgr in murine neutrophils. Taken together, these data demonstrate that CXCL1/MIP-2 leads to recruitment of neutrophils that, in turn, release biologically active VEGF-A, resulting in angiogenesis in vivo. Our observations delineate a novel mechanism by which CXCL1/MIP-2 induces neutrophil-dependent angiogenesis in vivo.

Original languageEnglish
Pages (from-to)5034-5040
Number of pages7
JournalJournal of Immunology
Volume172
Issue number8
Publication statusPublished - Apr 15 2004

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Chemokine CXCL2
Vascular Endothelial Growth Factor A
Neutrophils
Neutrophil Infiltration
Porifera
Interleukin-8
Chorioallantoic Membrane
CXC Chemokines
src-Family Kinases
Matrix Metalloproteinase 9
Chick Embryo
Oncogenes
Phosphotransferases

ASJC Scopus subject areas

  • Immunology

Cite this

Scapini, P., Morini, M., Tecchio, C., Minghelli, S., Di Carlo, E., Tanghetti, E., ... Cassatella, M. A. (2004). CXCL1/Macrophage Inflammatory Protein-2-Induced Angiogenesis In Vivo Is Mediated by Neutrophil-Derived Vascular Endothelial Growth Factor-A. Journal of Immunology, 172(8), 5034-5040.

CXCL1/Macrophage Inflammatory Protein-2-Induced Angiogenesis In Vivo Is Mediated by Neutrophil-Derived Vascular Endothelial Growth Factor-A. / Scapini, Patrizia; Morini, Monica; Tecchio, Cristina; Minghelli, Simona; Di Carlo, Emma; Tanghetti, Elena; Albini, Adriana; Lowell, Clifford; Berton, Giorgio; Noonan, Douglas M.; Cassatella, Marco A.

In: Journal of Immunology, Vol. 172, No. 8, 15.04.2004, p. 5034-5040.

Research output: Contribution to journalArticle

Scapini, P, Morini, M, Tecchio, C, Minghelli, S, Di Carlo, E, Tanghetti, E, Albini, A, Lowell, C, Berton, G, Noonan, DM & Cassatella, MA 2004, 'CXCL1/Macrophage Inflammatory Protein-2-Induced Angiogenesis In Vivo Is Mediated by Neutrophil-Derived Vascular Endothelial Growth Factor-A', Journal of Immunology, vol. 172, no. 8, pp. 5034-5040.
Scapini P, Morini M, Tecchio C, Minghelli S, Di Carlo E, Tanghetti E et al. CXCL1/Macrophage Inflammatory Protein-2-Induced Angiogenesis In Vivo Is Mediated by Neutrophil-Derived Vascular Endothelial Growth Factor-A. Journal of Immunology. 2004 Apr 15;172(8):5034-5040.
Scapini, Patrizia ; Morini, Monica ; Tecchio, Cristina ; Minghelli, Simona ; Di Carlo, Emma ; Tanghetti, Elena ; Albini, Adriana ; Lowell, Clifford ; Berton, Giorgio ; Noonan, Douglas M. ; Cassatella, Marco A. / CXCL1/Macrophage Inflammatory Protein-2-Induced Angiogenesis In Vivo Is Mediated by Neutrophil-Derived Vascular Endothelial Growth Factor-A. In: Journal of Immunology. 2004 ; Vol. 172, No. 8. pp. 5034-5040.
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abstract = "The angiogenic activity of CXC-ELR+ chemokines, including CXCL8/IL-8, CXCL1/macrophage inflammatory protein-2 (MIP-2), and CXCL1/growth-related oncogene-α in the Matrigel sponge angiogenesis assay in vivo, is strictly neutrophil dependent, as neutrophil depletion of the animals completely abrogates the angiogenic response. In this study, we demonstrate that mice deficient in the src family kinases, Hck and Fgr (hck -/-fgr-/-), are unable to develop an angiogenic response to CXCL1/MIP-2, although they respond normally to vascular endothelial growth factor-A (VEGF-A). Histological examination of the CXCL1/MIP-2-containing Matrigel implants isolated from wild-type or hck-/-fgr-/- mice showed the presence of an extensive neutrophil infiltrate, excluding a defective neutrophil recruitment into the Matrigel sponges. Accordingly, neutrophils from hck-/-fgr-/- mice normally migrated and released gelatinase B in response to CXCL1/MIP-2 in vitro, similarly to wild-type neutrophils. However, unlike wild-type neutrophils, those from hck-/-fgr-/- mice were completely unable to release VEGF-A upon stimulation with CXCL1/MIP-2. Furthermore, neutralizing anti-VEGF-A Abs abrogated the angiogenic response to CXCL1/MIP-2 in wild-type mice and CXCL1/MIP-2 induced angiogenesis in the chick embryo chorioallantoic membrane assay, indicating that neutrophil-derived VEGF-A is a major mediator of CXCL1/MIP-2-induced angiogenesis. Finally, in vitro kinase assays confirmed that CXCL1/MIP-2 activates Hck and Fgr in murine neutrophils. Taken together, these data demonstrate that CXCL1/MIP-2 leads to recruitment of neutrophils that, in turn, release biologically active VEGF-A, resulting in angiogenesis in vivo. Our observations delineate a novel mechanism by which CXCL1/MIP-2 induces neutrophil-dependent angiogenesis in vivo.",
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AU - Morini, Monica

AU - Tecchio, Cristina

AU - Minghelli, Simona

AU - Di Carlo, Emma

AU - Tanghetti, Elena

AU - Albini, Adriana

AU - Lowell, Clifford

AU - Berton, Giorgio

AU - Noonan, Douglas M.

AU - Cassatella, Marco A.

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