CXCR3-binding chemokines in multiple myeloma

Antonio Pellegrino, Francesco Antonaci, Francesca Russo, Francesca Merchionne, Domenico Ribatti, Angelo Vacca, Franco Dammacco

Research output: Contribution to journalArticlepeer-review

Abstract

The CXC chemokines I-TAC, Mig and IP10 and their receptor CXCR3 are associated with advanced-stage tumor and contribute to tumor progression, invasion and metastasis. The current study was designed to determine the expression of CXCR3 on four multiple myeloma (MM) cell lines and bone marrow plasma cells from 20 MM patients. Cell functions related to progression, such as tyrosine-kinase phosphorylation, proliferation, chemotaxis and matrix metalloproteinase-2 (MMP-2), and MMP-9 secretion were also investigated following the CXCR3/chemokine interaction. fluorescence activated cell sorting analysis revealed that three cell lines (75%) and 18 patients (90%) express the CXCR3 molecule. We demonstrated both in cell lines and fresh plasma cells that I-TAC, Mig and IP10 are able to induce tyrosine-kinase phosphorylation and chemotaxis, but not proliferation, and to increase the MMP-2 and MMP-9 gelatinolytic activity in the cell conditioned medium. Data suggest that CXCR3/chemokine loop may be important for progression of MM in terms of intramedullary and extramedullary dissemination.

Original languageEnglish
Pages (from-to)221-227
Number of pages7
JournalCancer Letters
Volume207
Issue number2
DOIs
Publication statusPublished - Apr 2004

Keywords

  • Chemokine-receptors
  • Chemokines
  • Metalloproteinases
  • Multiple myeloma

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

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