Cyanide binding to Lucina pectinata hemoglobin I and to sperm whale myoglobin: An x-ray crystallographic study

Martino Bolognesi, Camillo Rosano, Romeo Losso, Alberto Borassi, Menico Rizzi, Jonathan B. Wittenberg, Alberto Boffi, Paolo Ascenzi

Research output: Contribution to journalArticle

Abstract

The x-ray crystal structures of the cyanide derivative of Lucina pectinata monomeric hemoglobin I (L. pectinata Hbl) and sperm whale (Physeter catodon) myoglobin (Mb), generally taken as reference models for monomeric hemoproteins carrying hydrogen sulfide and oxygen, respectively, have been determined at 1.9 Å (R-factor = 0.184), and 1.8 Å (R-factor = 0.181) resolution, respectively, at room temperature (λ = 1.542 Å). Moreover, the x-ray crystal structure of the L. pectinata Hbl:cyanide derivative has been studied at 1.4-Å resolution (R-factor = 0.118) and 100 K (on a synchrotron source λ = 0.998 Å). At room temperature, the cyanide ligand is roughly parallel to the heme plane of L. pectinata Hbl, being located ~2.5 from the iron atom. On the other hand, the crystal structure of the L. pectinata Hbl:cyanide derivative at 100 K shows that the diatomic ligand is coordinated to the iron atom in an orientation almost perpendicular to the heme (the Fe-C distance being 1.95 Å), adopting a coordination geometry strictly reminescent of that observed in sperm whale Mb, at room temperature. The unusual cyanide distal site orientation observed in L. pectinata Hbl, at room temperature, may reflect reduction of the heme Fe(III) atom induced by free radical species during x-ray data collection using Cu Kα radiation.

Original languageEnglish
Pages (from-to)1093-1099
Number of pages7
JournalBiophysical Journal
Volume77
Issue number2
Publication statusPublished - Aug 1999

ASJC Scopus subject areas

  • Biophysics

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    Bolognesi, M., Rosano, C., Losso, R., Borassi, A., Rizzi, M., Wittenberg, J. B., Boffi, A., & Ascenzi, P. (1999). Cyanide binding to Lucina pectinata hemoglobin I and to sperm whale myoglobin: An x-ray crystallographic study. Biophysical Journal, 77(2), 1093-1099.