Cyanidin-3-O-β-glucoside and protocatechuic acid activate AMPK/mTOR/S6K pathway and improve glucose homeostasis in mice

Veena Talagavadi, Paolo Rapisarda, Fabio Galvano, Piergiuseppe Pelicci, Marco Giorgio

Research output: Contribution to journalArticle

Abstract

AMP-activated protein kinase (AMPK) activation is an established treatment for diabetes. Here the effects of anthocyanin extract from blood orange juice, i.e. cyanidin-3-O-β-glucoside (C3G) and its secondary metabolite protocatechuic acid (PCA), on AMPK signalling in murine hepatic cell line and in the liver of C57BL/6 mice were investigated. Results showed for the first time that C3G and PCA activate AMPK and suppress its downstream kinase mTOR/S6K both in vitro and in vivo systems. Then, C3G and particularly PCA increased expression of glut 1 and glut 4 in the liver and improved glucose tolerance in normal and obese mice. Finally, the consumption of blood orange juice was observed to increase insulin sensitivity in mice. These findings indicate that C3G and PCA are natural AMPK activators and dietary consumption of food sources of C3G and PCA improves glucose homeostasis. Mainly, blood orange juice has beneficial effect for type 2 diabetes.

Original languageEnglish
Pages (from-to)338-348
Number of pages11
JournalJournal of Functional Foods
Volume21
DOIs
Publication statusPublished - Mar 1 2016

Keywords

  • AMPK
  • Anthocyanin
  • Blood oranges
  • Glucose homeostasis
  • Laboratory mice
  • MTOR

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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