Cycle-dependent expression of interleukin-1 receptor type II in the human endometrium

A. Boucher, A. Kharfi, M. Al-Akoum, P. Bossù, A. Akoum

Research output: Contribution to journalArticle

Abstract

Cytokines such as interleukin-1 (IL-l) play a major role in the reparative and inflammatory-like processes that occur in human endometrium during every menstrual cycle, but they also seem to be implicated in critical reproductive events such as ovulation and implantation. Interleukin-1 is tightly regulated in the body by a complex network of control systems. In the present study, we examined the expression of IL-1RII, a natural specific inhibitor of IL-1, in the human endometrium and found an interesting distribution and temporal pattern of expression throughout the menstrual cycle. Immunoreactive IL-1RII was found in stromal as well as epithelial cells, but it was predominant within the lumen of the glands and the apical side of surface epithelium. In situ hybridization and reverse transcription-polymerase chain reaction (RT-PCR) analyses showed higher levels of mRNA in epithelial than in stromal cells. The IL-1RII cellular and luminal secretion followed a regulated cycle phase-dependent pattern of expression. Although elevated in the late proliferative/early secretory phase of the menstrual cycle, IL-1RII luminal secretion significantly decreased in the midsecretory phase, reaching its Iowest levels at Day 21, before augmenting markedly again during the late secretory phase. This pattern of expression was less obvious at the level of cellular staining, as examined by immunohistochemistry, but it was corroborated by Western blot analysis of IL-1RII protein and semiquantitative RT-PCR of IL-1RII mRNA in the whole endometrial tissue and separated glandular epithelial cells. The reduced expression of IL-1RII within the implantation window suggests the existence of accurate regulatory mechanisms that, by down-regulating IL-1RII expression, alleviate IL-1 inhibition during this crucial period and facilitate IL-1 proimplantation actions. The elevated expression of IL-1RII observed during the late secretory phase suggests an involvement of IL-1RII in control of the proinflammatory state that takes place in the endometrium during the premenstrual and menstrual periods.

Original languageEnglish
Pages (from-to)890-898
Number of pages9
JournalBiology of Reproduction
Volume65
Issue number3
Publication statusPublished - 2001

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Interleukin-1 Type II Receptors
Endometrium
Interleukin-1
Menstrual Cycle
Stromal Cells
Reverse Transcription
Epithelial Cells
Polymerase Chain Reaction
Messenger RNA
Luteal Phase
Ovulation
In Situ Hybridization
Epithelium
Western Blotting
Immunohistochemistry
human IL1R2 protein
Staining and Labeling
Cytokines

Keywords

  • Cytokines
  • Female reproductive tract
  • Menstrual cycle

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Cycle-dependent expression of interleukin-1 receptor type II in the human endometrium. / Boucher, A.; Kharfi, A.; Al-Akoum, M.; Bossù, P.; Akoum, A.

In: Biology of Reproduction, Vol. 65, No. 3, 2001, p. 890-898.

Research output: Contribution to journalArticle

Boucher, A, Kharfi, A, Al-Akoum, M, Bossù, P & Akoum, A 2001, 'Cycle-dependent expression of interleukin-1 receptor type II in the human endometrium', Biology of Reproduction, vol. 65, no. 3, pp. 890-898.
Boucher, A. ; Kharfi, A. ; Al-Akoum, M. ; Bossù, P. ; Akoum, A. / Cycle-dependent expression of interleukin-1 receptor type II in the human endometrium. In: Biology of Reproduction. 2001 ; Vol. 65, No. 3. pp. 890-898.
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AB - Cytokines such as interleukin-1 (IL-l) play a major role in the reparative and inflammatory-like processes that occur in human endometrium during every menstrual cycle, but they also seem to be implicated in critical reproductive events such as ovulation and implantation. Interleukin-1 is tightly regulated in the body by a complex network of control systems. In the present study, we examined the expression of IL-1RII, a natural specific inhibitor of IL-1, in the human endometrium and found an interesting distribution and temporal pattern of expression throughout the menstrual cycle. Immunoreactive IL-1RII was found in stromal as well as epithelial cells, but it was predominant within the lumen of the glands and the apical side of surface epithelium. In situ hybridization and reverse transcription-polymerase chain reaction (RT-PCR) analyses showed higher levels of mRNA in epithelial than in stromal cells. The IL-1RII cellular and luminal secretion followed a regulated cycle phase-dependent pattern of expression. Although elevated in the late proliferative/early secretory phase of the menstrual cycle, IL-1RII luminal secretion significantly decreased in the midsecretory phase, reaching its Iowest levels at Day 21, before augmenting markedly again during the late secretory phase. This pattern of expression was less obvious at the level of cellular staining, as examined by immunohistochemistry, but it was corroborated by Western blot analysis of IL-1RII protein and semiquantitative RT-PCR of IL-1RII mRNA in the whole endometrial tissue and separated glandular epithelial cells. The reduced expression of IL-1RII within the implantation window suggests the existence of accurate regulatory mechanisms that, by down-regulating IL-1RII expression, alleviate IL-1 inhibition during this crucial period and facilitate IL-1 proimplantation actions. The elevated expression of IL-1RII observed during the late secretory phase suggests an involvement of IL-1RII in control of the proinflammatory state that takes place in the endometrium during the premenstrual and menstrual periods.

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