Cyclin D1 expression is predictive of occult metastases in head and neck cancer patients with clinically negative cervical lymph nodes

Pasquale Capaccio, Giancarlo Pruneri, Nadia Carboni, Angelo Virgilio Pagliari, Massimiliano Quatela, Bruno Mario Cesana, Lorenzo Pignataro

Research output: Contribution to journalArticle

Abstract

Background. The aim of this study was to investigate the value of p53 and cyclin D1 gene expression in predicting the risk of occult lymph node metastases in patients with head and neck squamous cell carcinoma (HNSCC). Methods. The expression of cyclin D1 and p53 was evaluated by means of immunohistochemical analysis in 32 HNSCC patients with clinically and radiologically negative lymph nodes in whom metastatic involvement was subsequently demonstrated at histologic examination (pN+). A group of 64 head and neck cancer patients with histologically negative laterocervical lymph nodes (pN0) was used as a control. Results. Cyclin D1 and p53 expression were observed respectively in 42 (43.7%) and 48 cases (50%). Cyclin D1 expression significantly correlated with tumor extension and advanced clinical stage (p = .002 and p = .001, respectively). At univariate regression analysis, cyclin D1 expression significantly correlated with the presence of occult lymph node metastases (p = .0007), and it remained an independent predictor at multivariate regression analysis (p = .0059). Conclusions. Our study indicates that the expression of cyclin D1 correlates with the presence of occult cervical metastases in head and neck carcinoma patients, thus suggesting that its immunohistochemical evaluation in biopsy samples may be used as an additional tool for identifying patients to be treated with elective neck dissection. (C) 2000 John Wiley and Sons, Inc.

Original languageEnglish
Pages (from-to)234-240
Number of pages7
JournalHead and Neck
Volume22
Issue number3
DOIs
Publication statusPublished - May 2000

Fingerprint

Cyclin D1
Head and Neck Neoplasms
Lymph Nodes
Neoplasm Metastasis
Regression Analysis
bcl-1 Genes
Neck Dissection
Neck
Multivariate Analysis
Head
Carcinoma
Biopsy
Gene Expression
Neoplasms
Carcinoma, squamous cell of head and neck

Keywords

  • Cyclin D1
  • Head and neck cancer
  • Immunohistochemistry
  • Occult metastases
  • p53

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Cyclin D1 expression is predictive of occult metastases in head and neck cancer patients with clinically negative cervical lymph nodes. / Capaccio, Pasquale; Pruneri, Giancarlo; Carboni, Nadia; Pagliari, Angelo Virgilio; Quatela, Massimiliano; Cesana, Bruno Mario; Pignataro, Lorenzo.

In: Head and Neck, Vol. 22, No. 3, 05.2000, p. 234-240.

Research output: Contribution to journalArticle

Capaccio, Pasquale ; Pruneri, Giancarlo ; Carboni, Nadia ; Pagliari, Angelo Virgilio ; Quatela, Massimiliano ; Cesana, Bruno Mario ; Pignataro, Lorenzo. / Cyclin D1 expression is predictive of occult metastases in head and neck cancer patients with clinically negative cervical lymph nodes. In: Head and Neck. 2000 ; Vol. 22, No. 3. pp. 234-240.
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abstract = "Background. The aim of this study was to investigate the value of p53 and cyclin D1 gene expression in predicting the risk of occult lymph node metastases in patients with head and neck squamous cell carcinoma (HNSCC). Methods. The expression of cyclin D1 and p53 was evaluated by means of immunohistochemical analysis in 32 HNSCC patients with clinically and radiologically negative lymph nodes in whom metastatic involvement was subsequently demonstrated at histologic examination (pN+). A group of 64 head and neck cancer patients with histologically negative laterocervical lymph nodes (pN0) was used as a control. Results. Cyclin D1 and p53 expression were observed respectively in 42 (43.7{\%}) and 48 cases (50{\%}). Cyclin D1 expression significantly correlated with tumor extension and advanced clinical stage (p = .002 and p = .001, respectively). At univariate regression analysis, cyclin D1 expression significantly correlated with the presence of occult lymph node metastases (p = .0007), and it remained an independent predictor at multivariate regression analysis (p = .0059). Conclusions. Our study indicates that the expression of cyclin D1 correlates with the presence of occult cervical metastases in head and neck carcinoma patients, thus suggesting that its immunohistochemical evaluation in biopsy samples may be used as an additional tool for identifying patients to be treated with elective neck dissection. (C) 2000 John Wiley and Sons, Inc.",
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AU - Quatela, Massimiliano

AU - Cesana, Bruno Mario

AU - Pignataro, Lorenzo

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AB - Background. The aim of this study was to investigate the value of p53 and cyclin D1 gene expression in predicting the risk of occult lymph node metastases in patients with head and neck squamous cell carcinoma (HNSCC). Methods. The expression of cyclin D1 and p53 was evaluated by means of immunohistochemical analysis in 32 HNSCC patients with clinically and radiologically negative lymph nodes in whom metastatic involvement was subsequently demonstrated at histologic examination (pN+). A group of 64 head and neck cancer patients with histologically negative laterocervical lymph nodes (pN0) was used as a control. Results. Cyclin D1 and p53 expression were observed respectively in 42 (43.7%) and 48 cases (50%). Cyclin D1 expression significantly correlated with tumor extension and advanced clinical stage (p = .002 and p = .001, respectively). At univariate regression analysis, cyclin D1 expression significantly correlated with the presence of occult lymph node metastases (p = .0007), and it remained an independent predictor at multivariate regression analysis (p = .0059). Conclusions. Our study indicates that the expression of cyclin D1 correlates with the presence of occult cervical metastases in head and neck carcinoma patients, thus suggesting that its immunohistochemical evaluation in biopsy samples may be used as an additional tool for identifying patients to be treated with elective neck dissection. (C) 2000 John Wiley and Sons, Inc.

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