TY - JOUR
T1 - Cyclin D3 immunoreactivity is an independent predictor of survival in laryngeal squamous cell carcinoma
AU - Pruneri, Giancarlo
AU - Pignataro, Lorenzo
AU - Valentini, Stefano
AU - Fabris, Sonia
AU - Maisonneuve, Patrick
AU - Carboni, Nadia
AU - Pece, Salvatore
AU - Capra, Maria
AU - Del Curto, Barbara
AU - Neri, Antonino
AU - Viale, Giuseppe
PY - 2005/1/1
Y1 - 2005/1/1
N2 - Purpose: To analyze the prevalence and clinical relevance of cyclin D3 abnormalities in laryngeal squamous cell carcinoma (LSCC). Experimental Design: Cyclin D3 immunoreactivity was evaluated in 223 formalin-fixed and paraffin-embedded samples of LSCC patients with a mean follow-up of 62.8 ± 43.2 months. The occurrence of cyclin D3 extra signals was analyzed by fluorescence in situ hybridization in 47 randomly selected cases collected in a tissue microarray. Cyclin D1 immunoreactivity had been previously investigated in 133 cases. Results: Cyclin D3 immunoreactivity and gene extra signals were found in 39.5% and 42.6% of the cases, respectively, and the concordance between immunohistochemical and fluorescence in situ hybridization results was 70.2% (P = 0.0085). Cyclin D3 immunoreactivity was significantly associated with a high risk of death. Multivariate analysis showed that high tumor grade, exophytic/ulcerating tumor type, low performance status, and cyclin D3 immunoreactivity were the only independent predictors of poor overall survival. In the 133 cases analyzed for both cyclin D1 and cyclin D3, patients with cyclin D1 +/cyclin D3 + tumors experienced the worst prognosis, patients with cyclin D1 -/ cyclin D3 - exhibited the most prolonged survival, and with cyclin D1 -/cyclin D3 + or cyclin D1 +/cyc!in D3 - tumors an intermediate course was associated. Conclusions: Our data suggest that cyclim D3 immunoreactivity, possibly due to the occurrence of gene extra copies, may represent an adjunct in LSCC patients' prognostication and contribute to identify D-type cyclins as potential targets of newly developed therapies.
AB - Purpose: To analyze the prevalence and clinical relevance of cyclin D3 abnormalities in laryngeal squamous cell carcinoma (LSCC). Experimental Design: Cyclin D3 immunoreactivity was evaluated in 223 formalin-fixed and paraffin-embedded samples of LSCC patients with a mean follow-up of 62.8 ± 43.2 months. The occurrence of cyclin D3 extra signals was analyzed by fluorescence in situ hybridization in 47 randomly selected cases collected in a tissue microarray. Cyclin D1 immunoreactivity had been previously investigated in 133 cases. Results: Cyclin D3 immunoreactivity and gene extra signals were found in 39.5% and 42.6% of the cases, respectively, and the concordance between immunohistochemical and fluorescence in situ hybridization results was 70.2% (P = 0.0085). Cyclin D3 immunoreactivity was significantly associated with a high risk of death. Multivariate analysis showed that high tumor grade, exophytic/ulcerating tumor type, low performance status, and cyclin D3 immunoreactivity were the only independent predictors of poor overall survival. In the 133 cases analyzed for both cyclin D1 and cyclin D3, patients with cyclin D1 +/cyclin D3 + tumors experienced the worst prognosis, patients with cyclin D1 -/ cyclin D3 - exhibited the most prolonged survival, and with cyclin D1 -/cyclin D3 + or cyclin D1 +/cyc!in D3 - tumors an intermediate course was associated. Conclusions: Our data suggest that cyclim D3 immunoreactivity, possibly due to the occurrence of gene extra copies, may represent an adjunct in LSCC patients' prognostication and contribute to identify D-type cyclins as potential targets of newly developed therapies.
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M3 - Article
C2 - 15671552
AN - SCOPUS:19944429941
VL - 11
SP - 242
EP - 248
JO - Clinical Cancer Research
JF - Clinical Cancer Research
SN - 1078-0432
IS - 1
ER -