Cycloheximide can rescue heat-shocked L cells from death by blocking stress-induced apoptosis

L. Ghibelli, C. Nosseri, S. Oliverio, M. Piacentini, F. Autuori

Research output: Contribution to journalArticlepeer-review

Abstract

Cultured mouse L cells undergo apoptosis upon 1 h heat shock at 43 and 45 °C. Morphologically characteristic apoptotic cells begin to appear soon after the shock. Immunohistochemistry with anti-transglutaminase antibody shows that in most treated cells the enzyme is induced. Its activation results in the formation of highly cross-linked detergent-resistant apoptotic bodies during recovery. Cycloheximide added during hyperthermic stress inhibits the appearance of apoptotic bodies, showing that heat-shock-induced apoptosis is dependent on protein neosynthesis. The analysis of colony-forming ability of heat-shocked L cells shows a survival of 5% at 43 °C and

Original languageEnglish
Pages (from-to)436-443
Number of pages8
JournalExperimental Cell Research
Volume201
Issue number2
DOIs
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Cell Biology

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