Cycloheximide can rescue heat-shocked L cells from death by blocking stress-induced apoptosis

L. Ghibelli; C. Nosseri; S. Oliverio; M. Piacentini; F. Autuori

doi:10.1016/0014-4827(92)90292-G

Cycloheximide can rescue heat-shocked L cells from death by blocking stress-induced apoptosis

L. Ghibelli, C. Nosseri, S. Oliverio, M. Piacentini, F. Autuori

Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani

Research output: Contribution to journal › Article › peer-review

Abstract

Cultured mouse L cells undergo apoptosis upon 1 h heat shock at 43 and 45 °C. Morphologically characteristic apoptotic cells begin to appear soon after the shock. Immunohistochemistry with anti-transglutaminase antibody shows that in most treated cells the enzyme is induced. Its activation results in the formation of highly cross-linked detergent-resistant apoptotic bodies during recovery. Cycloheximide added during hyperthermic stress inhibits the appearance of apoptotic bodies, showing that heat-shock-induced apoptosis is dependent on protein neosynthesis. The analysis of colony-forming ability of heat-shocked L cells shows a survival of 5% at 43 °C and

Original language	English
Pages (from-to)	436-443
Number of pages	8
Journal	Experimental Cell Research
Volume	201
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(92)90292-G
Publication status	Published - 1992

ASJC Scopus subject areas

Cell Biology

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title = "Cycloheximide can rescue heat-shocked L cells from death by blocking stress-induced apoptosis",

abstract = "Cultured mouse L cells undergo apoptosis upon 1 h heat shock at 43 and 45 °C. Morphologically characteristic apoptotic cells begin to appear soon after the shock. Immunohistochemistry with anti-transglutaminase antibody shows that in most treated cells the enzyme is induced. Its activation results in the formation of highly cross-linked detergent-resistant apoptotic bodies during recovery. Cycloheximide added during hyperthermic stress inhibits the appearance of apoptotic bodies, showing that heat-shock-induced apoptosis is dependent on protein neosynthesis. The analysis of colony-forming ability of heat-shocked L cells shows a survival of 5% at 43 °C and ",

author = "L. Ghibelli and C. Nosseri and S. Oliverio and M. Piacentini and F. Autuori",

year = "1992",

doi = "10.1016/0014-4827(92)90292-G",

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T1 - Cycloheximide can rescue heat-shocked L cells from death by blocking stress-induced apoptosis

AU - Ghibelli, L.

AU - Nosseri, C.

AU - Oliverio, S.

AU - Piacentini, M.

AU - Autuori, F.

PY - 1992

Y1 - 1992

N2 - Cultured mouse L cells undergo apoptosis upon 1 h heat shock at 43 and 45 °C. Morphologically characteristic apoptotic cells begin to appear soon after the shock. Immunohistochemistry with anti-transglutaminase antibody shows that in most treated cells the enzyme is induced. Its activation results in the formation of highly cross-linked detergent-resistant apoptotic bodies during recovery. Cycloheximide added during hyperthermic stress inhibits the appearance of apoptotic bodies, showing that heat-shock-induced apoptosis is dependent on protein neosynthesis. The analysis of colony-forming ability of heat-shocked L cells shows a survival of 5% at 43 °C and

AB - Cultured mouse L cells undergo apoptosis upon 1 h heat shock at 43 and 45 °C. Morphologically characteristic apoptotic cells begin to appear soon after the shock. Immunohistochemistry with anti-transglutaminase antibody shows that in most treated cells the enzyme is induced. Its activation results in the formation of highly cross-linked detergent-resistant apoptotic bodies during recovery. Cycloheximide added during hyperthermic stress inhibits the appearance of apoptotic bodies, showing that heat-shock-induced apoptosis is dependent on protein neosynthesis. The analysis of colony-forming ability of heat-shocked L cells shows a survival of 5% at 43 °C and

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Cycloheximide can rescue heat-shocked L cells from death by blocking stress-induced apoptosis

Abstract

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