Abstract
Cultured mouse L cells undergo apoptosis upon 1 h heat shock at 43 and 45 °C. Morphologically characteristic apoptotic cells begin to appear soon after the shock. Immunohistochemistry with anti-transglutaminase antibody shows that in most treated cells the enzyme is induced. Its activation results in the formation of highly cross-linked detergent-resistant apoptotic bodies during recovery. Cycloheximide added during hyperthermic stress inhibits the appearance of apoptotic bodies, showing that heat-shock-induced apoptosis is dependent on protein neosynthesis. The analysis of colony-forming ability of heat-shocked L cells shows a survival of 5% at 43 °C and
Original language | English |
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Pages (from-to) | 436-443 |
Number of pages | 8 |
Journal | Experimental Cell Research |
Volume | 201 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1992 |
ASJC Scopus subject areas
- Cell Biology