Cycloheximide inhibits kainic acid-induced GAP-43 mRNA in dentate granule cells in rats

Caterina Bendotti, Mario Pende, Filippo Guglielmetti, Rosario Samanin

Research output: Contribution to journalArticle

Abstract

We have previously shown that kainic acid-induced seizures in adult rats caused an up-regulation of GAP-43 mRNA in the granule cells of the hippocampus, suggesting an involvement of this protein in the kainic acid-induced sprouting of messy fibres. To determine whether this effect was dependent on the synthesis of proteins activated under these experimental conditions we examined the effect of cycloheximide, a protein synthesis inhibitor, on kainic acid-induced GAP-43 mRNA. Cycloheximide, injected s.c. 2 h but not 8 h after kainic acid, markedly reduced the increased expression of GAP-43 mRNA in granule cells. These results suggest that a rapid mechanism involving new protein synthesis is activated by kainic acid to induce GAP-43 in the granule cells and possibly trigger the structural remodeling of messy fibres.

Original languageEnglish
Pages (from-to)2539-2542
Number of pages4
JournalNeuroReport
Volume7
Issue number15-17
Publication statusPublished - 1996

Keywords

  • Cycloheximide
  • Dentate messy fibres
  • Epilepsy
  • GAP-43
  • Hippocampus
  • In situ hybridization
  • Kainic acid
  • Sprouting

ASJC Scopus subject areas

  • Neuroscience(all)

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    Bendotti, C., Pende, M., Guglielmetti, F., & Samanin, R. (1996). Cycloheximide inhibits kainic acid-induced GAP-43 mRNA in dentate granule cells in rats. NeuroReport, 7(15-17), 2539-2542.