Cycloheximide inhibits kainic acid-induced GAP-43 mRNA in dentate granule cells in rats

Caterina Bendotti, Mario Pende, Filippo Guglielmetti, Rosario Samanin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We have previously shown that kainic acid-induced seizures in adult rats caused an up-regulation of GAP-43 mRNA in the granule cells of the hippocampus, suggesting an involvement of this protein in the kainic acid-induced sprouting of messy fibres. To determine whether this effect was dependent on the synthesis of proteins activated under these experimental conditions we examined the effect of cycloheximide, a protein synthesis inhibitor, on kainic acid-induced GAP-43 mRNA. Cycloheximide, injected s.c. 2 h but not 8 h after kainic acid, markedly reduced the increased expression of GAP-43 mRNA in granule cells. These results suggest that a rapid mechanism involving new protein synthesis is activated by kainic acid to induce GAP-43 in the granule cells and possibly trigger the structural remodeling of messy fibres.

Original languageEnglish
Pages (from-to)2539-2542
Number of pages4
JournalNeuroReport
Volume7
Issue number15-17
Publication statusPublished - 1996

Fingerprint

GAP-43 Protein
Kainic Acid
Cycloheximide
Messenger RNA
Proteins
Protein Synthesis Inhibitors
Hippocampus
Seizures
Up-Regulation

Keywords

  • Cycloheximide
  • Dentate messy fibres
  • Epilepsy
  • GAP-43
  • Hippocampus
  • In situ hybridization
  • Kainic acid
  • Sprouting

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Bendotti, C., Pende, M., Guglielmetti, F., & Samanin, R. (1996). Cycloheximide inhibits kainic acid-induced GAP-43 mRNA in dentate granule cells in rats. NeuroReport, 7(15-17), 2539-2542.

Cycloheximide inhibits kainic acid-induced GAP-43 mRNA in dentate granule cells in rats. / Bendotti, Caterina; Pende, Mario; Guglielmetti, Filippo; Samanin, Rosario.

In: NeuroReport, Vol. 7, No. 15-17, 1996, p. 2539-2542.

Research output: Contribution to journalArticle

Bendotti, C, Pende, M, Guglielmetti, F & Samanin, R 1996, 'Cycloheximide inhibits kainic acid-induced GAP-43 mRNA in dentate granule cells in rats', NeuroReport, vol. 7, no. 15-17, pp. 2539-2542.
Bendotti, Caterina ; Pende, Mario ; Guglielmetti, Filippo ; Samanin, Rosario. / Cycloheximide inhibits kainic acid-induced GAP-43 mRNA in dentate granule cells in rats. In: NeuroReport. 1996 ; Vol. 7, No. 15-17. pp. 2539-2542.
@article{63ab7cbe96334f4cbf066dbd5ae1667e,
title = "Cycloheximide inhibits kainic acid-induced GAP-43 mRNA in dentate granule cells in rats",
abstract = "We have previously shown that kainic acid-induced seizures in adult rats caused an up-regulation of GAP-43 mRNA in the granule cells of the hippocampus, suggesting an involvement of this protein in the kainic acid-induced sprouting of messy fibres. To determine whether this effect was dependent on the synthesis of proteins activated under these experimental conditions we examined the effect of cycloheximide, a protein synthesis inhibitor, on kainic acid-induced GAP-43 mRNA. Cycloheximide, injected s.c. 2 h but not 8 h after kainic acid, markedly reduced the increased expression of GAP-43 mRNA in granule cells. These results suggest that a rapid mechanism involving new protein synthesis is activated by kainic acid to induce GAP-43 in the granule cells and possibly trigger the structural remodeling of messy fibres.",
keywords = "Cycloheximide, Dentate messy fibres, Epilepsy, GAP-43, Hippocampus, In situ hybridization, Kainic acid, Sprouting",
author = "Caterina Bendotti and Mario Pende and Filippo Guglielmetti and Rosario Samanin",
year = "1996",
language = "English",
volume = "7",
pages = "2539--2542",
journal = "NeuroReport",
issn = "0959-4965",
publisher = "Lippincott Williams and Wilkins",
number = "15-17",

}

TY - JOUR

T1 - Cycloheximide inhibits kainic acid-induced GAP-43 mRNA in dentate granule cells in rats

AU - Bendotti, Caterina

AU - Pende, Mario

AU - Guglielmetti, Filippo

AU - Samanin, Rosario

PY - 1996

Y1 - 1996

N2 - We have previously shown that kainic acid-induced seizures in adult rats caused an up-regulation of GAP-43 mRNA in the granule cells of the hippocampus, suggesting an involvement of this protein in the kainic acid-induced sprouting of messy fibres. To determine whether this effect was dependent on the synthesis of proteins activated under these experimental conditions we examined the effect of cycloheximide, a protein synthesis inhibitor, on kainic acid-induced GAP-43 mRNA. Cycloheximide, injected s.c. 2 h but not 8 h after kainic acid, markedly reduced the increased expression of GAP-43 mRNA in granule cells. These results suggest that a rapid mechanism involving new protein synthesis is activated by kainic acid to induce GAP-43 in the granule cells and possibly trigger the structural remodeling of messy fibres.

AB - We have previously shown that kainic acid-induced seizures in adult rats caused an up-regulation of GAP-43 mRNA in the granule cells of the hippocampus, suggesting an involvement of this protein in the kainic acid-induced sprouting of messy fibres. To determine whether this effect was dependent on the synthesis of proteins activated under these experimental conditions we examined the effect of cycloheximide, a protein synthesis inhibitor, on kainic acid-induced GAP-43 mRNA. Cycloheximide, injected s.c. 2 h but not 8 h after kainic acid, markedly reduced the increased expression of GAP-43 mRNA in granule cells. These results suggest that a rapid mechanism involving new protein synthesis is activated by kainic acid to induce GAP-43 in the granule cells and possibly trigger the structural remodeling of messy fibres.

KW - Cycloheximide

KW - Dentate messy fibres

KW - Epilepsy

KW - GAP-43

KW - Hippocampus

KW - In situ hybridization

KW - Kainic acid

KW - Sprouting

UR - http://www.scopus.com/inward/record.url?scp=0029827794&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029827794&partnerID=8YFLogxK

M3 - Article

C2 - 8981419

AN - SCOPUS:0029827794

VL - 7

SP - 2539

EP - 2542

JO - NeuroReport

JF - NeuroReport

SN - 0959-4965

IS - 15-17

ER -