Cyclooxygenase-dependent thyroid cell proliferation induced by immunoglobulins from patients with Graves' disease

Rosa Di Paola, Claudia Menzaghi, Vito De Filippis, Daniela Corda, Alfredo Di Cerbo

Research output: Contribution to journalArticlepeer-review

Abstract

IgG associated with Graves' disease bind to the TSH receptor and alter thyroid growth and function, mainly through the stimulation of adenylyl cyclase. In addition, Graves' IgG are able to interact with the phospholipase C (PLC)/Ca2+ and phospholipase A2 (PLA2)/arachidonic acid (AA) cascades. The activation of this latter pathway leads to thyroid cell growth in vitro. The elucidation of additional mechanisms of action of Graves' IgG has made possible the identification of four subgroups of patients, characterized by IgG with different biochemical activities (extent of cAMP and AA release stimulation in in vitro assays). On the basis of these results, a novel therapeutic approach could be proposed based on the inhibition of PLA2 and AA metabolism. To test this hypothesis, the ability of IgG from 56 Graves' patients to stimulate [3H]thymidine incorporation in FRTL5 thyroid cells in the presence and absence of the cyclooxygenase inhibitor indomethacin (2.5 x 10-6 mol/L) was measured. A significant reduction in [3H]thymidine incorporation was found (33% inhibition; P <0.0061) upon pretreatment with indomethacin, suggesting that in vitro thyroid cell growth is regulated by cyclooxygenase metabolites. This strengthens the argument for involvement of the PLA2/AA cascade in the pathophysiology of Graves' disease and the proposal for novel selective pharmacological treatments of these patients.

Original languageEnglish
Pages (from-to)670-673
Number of pages4
JournalJournal of Clinical Endocrinology and Metabolism
Volume82
Issue number2
DOIs
Publication statusPublished - 1997

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

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