Cyclosporin A (CyA) does not enhance CFU-c growth in patients with severe aplastic anaemia

A. Bacigalupo, F. Frassoni, M. Podesta', G. Piaggio, M. T. Van Lint, M. Repetto, A. M. Marmont

Research output: Contribution to journalArticle

Abstract

8 patients with severe aplastic anaemia (SAA) in remission following immunosuppressive therapy were studied for CFU-c growth from unfractionated or E-rosette depleted (E-) bone marrow (BM) cells. Cyclosporin A (CyA) was added to unfractionated BM cells at a concentration of 1000 ng/ml. The mean number of CFU-c/105 BM cells plated was 6 ± 6 from unfractionated BM cells, 28 ± 20 from E-BM cells, and 8 ± 7 from unfractionated BM cells supplemented with CyA. All patients had significant increase of CFU-c growth after E rosette depletion (overall P = 0.002). On the contrary, only 1 patient showed an increase of CFU-c growth after addition of CyA, and overall there was no difference between untreated and CyA treated BM cells (P = 0.7). These results suggest that addition of CyA to BM cells in vitro is not an effective means of enhancing CFU-c growth in SAA patients.

Original languageEnglish
Pages (from-to)133-136
Number of pages4
JournalScandinavian Journal of Haematology
Volume34
Issue number2
Publication statusPublished - 1985

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Aplastic Anemia
Bone Marrow Cells
Cyclosporine
Growth
Immunosuppressive Agents

ASJC Scopus subject areas

  • Hematology

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Cyclosporin A (CyA) does not enhance CFU-c growth in patients with severe aplastic anaemia. / Bacigalupo, A.; Frassoni, F.; Podesta', M.; Piaggio, G.; Van Lint, M. T.; Repetto, M.; Marmont, A. M.

In: Scandinavian Journal of Haematology, Vol. 34, No. 2, 1985, p. 133-136.

Research output: Contribution to journalArticle

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abstract = "8 patients with severe aplastic anaemia (SAA) in remission following immunosuppressive therapy were studied for CFU-c growth from unfractionated or E-rosette depleted (E-) bone marrow (BM) cells. Cyclosporin A (CyA) was added to unfractionated BM cells at a concentration of 1000 ng/ml. The mean number of CFU-c/105 BM cells plated was 6 ± 6 from unfractionated BM cells, 28 ± 20 from E-BM cells, and 8 ± 7 from unfractionated BM cells supplemented with CyA. All patients had significant increase of CFU-c growth after E rosette depletion (overall P = 0.002). On the contrary, only 1 patient showed an increase of CFU-c growth after addition of CyA, and overall there was no difference between untreated and CyA treated BM cells (P = 0.7). These results suggest that addition of CyA to BM cells in vitro is not an effective means of enhancing CFU-c growth in SAA patients.",
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T1 - Cyclosporin A (CyA) does not enhance CFU-c growth in patients with severe aplastic anaemia

AU - Bacigalupo, A.

AU - Frassoni, F.

AU - Podesta', M.

AU - Piaggio, G.

AU - Van Lint, M. T.

AU - Repetto, M.

AU - Marmont, A. M.

PY - 1985

Y1 - 1985

N2 - 8 patients with severe aplastic anaemia (SAA) in remission following immunosuppressive therapy were studied for CFU-c growth from unfractionated or E-rosette depleted (E-) bone marrow (BM) cells. Cyclosporin A (CyA) was added to unfractionated BM cells at a concentration of 1000 ng/ml. The mean number of CFU-c/105 BM cells plated was 6 ± 6 from unfractionated BM cells, 28 ± 20 from E-BM cells, and 8 ± 7 from unfractionated BM cells supplemented with CyA. All patients had significant increase of CFU-c growth after E rosette depletion (overall P = 0.002). On the contrary, only 1 patient showed an increase of CFU-c growth after addition of CyA, and overall there was no difference between untreated and CyA treated BM cells (P = 0.7). These results suggest that addition of CyA to BM cells in vitro is not an effective means of enhancing CFU-c growth in SAA patients.

AB - 8 patients with severe aplastic anaemia (SAA) in remission following immunosuppressive therapy were studied for CFU-c growth from unfractionated or E-rosette depleted (E-) bone marrow (BM) cells. Cyclosporin A (CyA) was added to unfractionated BM cells at a concentration of 1000 ng/ml. The mean number of CFU-c/105 BM cells plated was 6 ± 6 from unfractionated BM cells, 28 ± 20 from E-BM cells, and 8 ± 7 from unfractionated BM cells supplemented with CyA. All patients had significant increase of CFU-c growth after E rosette depletion (overall P = 0.002). On the contrary, only 1 patient showed an increase of CFU-c growth after addition of CyA, and overall there was no difference between untreated and CyA treated BM cells (P = 0.7). These results suggest that addition of CyA to BM cells in vitro is not an effective means of enhancing CFU-c growth in SAA patients.

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