Nitric oxide (NO) may modulate the catalytic activity of cysteine proteases. In the present study, the inhibitory effect of NO, released by the NO-donors (±)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide and nitroprusside, on papain action is reported. Papain inactivation via NO-mediated nitrosylation of the Cys25 catalytic residue represents a molecular model for cysteine protease inhibition.
|Number of pages||4|
|Journal||Biochemistry and Molecular Biology International|
|Publication status||Published - 1998|
- Enzyme inhibition
ASJC Scopus subject areas
- Molecular Biology