Cys25-nitrosylation inactivates papain

Giorgio Venturini, Emanuela Fioravanti, Marco Colasanti, Tiziana Persichini, Paolo Ascenzi

Research output: Contribution to journalArticlepeer-review


Nitric oxide (NO) may modulate the catalytic activity of cysteine proteases. In the present study, the inhibitory effect of NO, released by the NO-donors (±)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide and nitroprusside, on papain action is reported. Papain inactivation via NO-mediated nitrosylation of the Cys25 catalytic residue represents a molecular model for cysteine protease inhibition.

Original languageEnglish
Pages (from-to)425-428
Number of pages4
JournalBiochemistry and Molecular Biology International
Issue number2
Publication statusPublished - 1998


  • Cys25-nitrosylation
  • Enzyme inhibition
  • Papain

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology


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