Cytochemical analysis of peripheral blood mononuclear cells following allogeneic bone marrow transplantation: Correlation of hydrolase expression with graft-versus-host disease

M. Repetto, A. Bacigalupo, M. Viale, G. Piaggio, M. Gandini, F. Frassoni, A. M. Marmont, M. T. Van Lint

Research output: Contribution to journalArticle

Abstract

Peripheral blood mononuclear cells of 21 patients undergoing allogeneic bone marrow transplantation (BMT) were monitored post-BMT for immunologic markers (E rosettes OKT3, OKT8, DR, and BT5/9, a monoclonal antibody which stains helper T cells), cytochemical markers (acid phosphatase [AP], β-glucuronidase [BGLU], and acid alpha naphthyl acetate esterase [ANAE], and morphology. The cytochemical T score, was obtained from typical AP, BGLU, and ANAE reactivity. The same was done for the cytochemical non-T score and macrophage (Mo) score. All patients received cyclosporine A (CyA) for graft-v-host disease (GvHD) prophylaxis. In univariate analysis there was no significant correlation between the proportion of E rosettes, OKT3-, OKT8-, DR-, and BT5/9-positive cells, and GvHD. The first three showed instead a positive correlation with time from transplant: E rosettes (P=.02), OKT3 (P=.01), and OKT8 (P=.003). In contrast, a significant negative correlation was found in univariate analysis, between the cytochemical T score and GvHD (P=.0001), and a positive correlation between non-T score and GvHD (P=.0008), as well as between the Mo score and GvHD (P=.03). There was no influence of time from transplant on the T (P=.8), non-T (P=.8), or Mo score (P=.4). In multivariate analysis comparing E rosettes, OKT3, T score, non-T score, GvHD, and time from BMT, the only variable associated with GvHD was the T score (P

Original languageEnglish
Pages (from-to)1011-1016
Number of pages6
JournalBlood
Volume66
Issue number5
Publication statusPublished - 1985

ASJC Scopus subject areas

  • Hematology

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