TY - JOUR
T1 - Cytochemical estimation of cytochrome oxidase activity as a morphofunctional mitochondrial check-up
AU - Bertoni-Freddari, Carlo
AU - Fattoretti, Patrizia
AU - Giorgetti, Belinda
AU - Grossi, Yessica
AU - Casoli, Tiziana
AU - Di Stefano, Giuseppina
AU - Balietti, Marta
PY - 2006/6
Y1 - 2006/6
N2 - Cytochemical estimation of COX activity and morphometric measurement of mitochondrial ultrastructure were carried out in organelles from adult and old rats. Although no age-related difference was found in cytochemical precipitate (CPA) and mitochondrial area (MA), the ratio CPA:MA (R) decreased by 25.7% in aging. R was the same in oversized mitochondria, but in smaller organelles it was significantly decreased during aging. R reports on the functional mitochondrial surface involved in energy providing mechanisms; thus, by revealing age-related differences, these data suggest that the COX preferential cytochemistry associated with morphometry may serve as a reliable and sensitive mitochondrial morphofunctional check-up procedure.
AB - Cytochemical estimation of COX activity and morphometric measurement of mitochondrial ultrastructure were carried out in organelles from adult and old rats. Although no age-related difference was found in cytochemical precipitate (CPA) and mitochondrial area (MA), the ratio CPA:MA (R) decreased by 25.7% in aging. R was the same in oversized mitochondria, but in smaller organelles it was significantly decreased during aging. R reports on the functional mitochondrial surface involved in energy providing mechanisms; thus, by revealing age-related differences, these data suggest that the COX preferential cytochemistry associated with morphometry may serve as a reliable and sensitive mitochondrial morphofunctional check-up procedure.
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U2 - 10.1089/rej.2006.9.202
DO - 10.1089/rej.2006.9.202
M3 - Article
C2 - 16706643
AN - SCOPUS:33744953978
VL - 9
SP - 202
EP - 206
JO - Rejuvenation Research
JF - Rejuvenation Research
SN - 1549-1684
IS - 2
ER -