Studio citochimico sul metabolismo degli spermatozoi umani nel corso della capacitazione in vitro anche dopo crioconservazione.

Translated title of the contribution: Cytochemical study of human sperm metabolism during in vitro capacitation after cryopreservation

A. Lange Consiglio, F. Chiara, M. Marchini, A. Baglioni, L. Falcone, A. Carnevali, F. Minoletti, F. Cremonesi

Research output: Contribution to journalArticlepeer-review

Abstract

To study the effects of deep freezing on the energy metabolism of human spermatozoa, we investigated, by cytochemical quantitative methods, cytochrome oxidase and lactate dehydrogenase activities of fresh and frozen human spermatozoa during in vitro capacitation. Fresh and frozen human spermatozoa were incubated in Biggers, Whitten and Wittingham's medium supplemented with 15% heat-inactivated human serum. Both histoenzymological reactions can be quantitated and have been evaluated by microdensitometric method. The results indicate that human spermatozoa depend almost entirely on anaerobic glycolysis during in vitro capacitation and suggest that both aerobic and anaerobic metabolism in spermatozoa are only slightly impaired by freezing-thawing and storage.

Translated title of the contributionCytochemical study of human sperm metabolism during in vitro capacitation after cryopreservation
Original languageItalian
Pages (from-to)115-122
Number of pages8
JournalArchivio italiano di urologia, nefrologia, andrologia : organo ufficiale dell"Associazione per la ricerca in urologia = Urological, nephrological, and andrological sciences
Volume64
Issue number2
Publication statusPublished - Jun 1992

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint Dive into the research topics of 'Cytochemical study of human sperm metabolism during in vitro capacitation after cryopreservation'. Together they form a unique fingerprint.

Cite this