Flow cytometric study of the ploidy is employed to quantify modifications of the normal cell DNA content. Cytofluorimetric analysis allows the detection in tissue samples of the eventual presence of cellular clones with anomalous DNA content and the differentiation of diploid from aneuploid populations. Although flow cytometry evidences only > 3% quantitative modifications of DNA content, the study of the ploidy has been carried out in many tumors to estimate the clinical value of the obtained data. Generally, aneuploid tumors are more aggressive even if the prognostic role of the ploidy is not completely defined for all the types of neoplasia. In the present article we describe some of the principles on which cytofluorimetric analysis is based as well as the applications of the ploidy study in some pediatric tumors (Ewing sarcoma, rhabdomyosarcoma, Wilms' tumor). More in detail, we report a study of DNA content in a group of 143 cases of localized neuroblastoma carried out in the Pathology Department of Gaslini Institute. In neuroblastic tumors, although there are other important prognostic factors, DNA content plays a remarkable role since the presence of aneuploid clones seems to be associated to better survival. In our study the values of the ploidy were compared to histological data to verify if DNA index has a real prognostic value in the histotypes in which prognosis formulation is more difficult. Our data suggest that the cytofluorimetric study of DNA content in neuroblastic tumors has a prognostic importance only in "Schwannian stroma poor" neuroblastoma; therefore, cytofluorimetric analysis of DNA content in neuroblastic tumors, if supported by histology, gives an important contribution to prediction of recurrence or persistence of disease.
|Translated title of the contribution||Cytofluorimetric analysis of DNA content in normal and neoplastic tissue|
|Number of pages||7|
|Publication status||Published - 2001|
ASJC Scopus subject areas
- Pediatrics, Perinatology, and Child Health