Cytofluorimetric and immunohistochemical comparison for detecting bone marrow infiltration in non-Hodgkin lymphomas: a study of 354 patients: Leukemia Research

T. Statuto, L. Valvano, G. Calice, O. Villani, G. Pietrantuono, G. Mansueto, G. D'Arena, G. Vita, V. Lalinga, L. Possidente, L. Del Vecchio, F. D'Auria, P. Musto

Research output: Contribution to journalArticlepeer-review


Morphological and immunohistochemical (IHC) analysis of bone marrow biopsies (BMB) is routinely performed during staging of patients with non-Hodgkin's lymphoma (NHL). Aiming to evaluate the possible diagnostic value of flow cytometry (FC) on bone marrow aspirates (BMA), as compared with BMB, we retrospectively reviewed BMA specimen of 354 NHL. In 305 cases (86.1 %), there was a concordance between the two investigations. A discordance was detected in 49 cases (14 %): in 33 of these (9.3 % of total population), FC analysis of BMA was positive, whereas BMB, supported by IHC, was negative; in 16 (4.5 % of total population), FC did not detected lymphoid infiltration, while BMB was positive. Although the clinical implications of such an observation remain unclear, we think our results may be useful in the context of current staging procedures, also opening a possible future perspective in the setting of minimal measurable disease in these patients. © 2019
Original languageEnglish
JournalLeuk. Res.
Publication statusPublished - 2020


  • Bone marrow infiltration
  • Flow cytometry
  • Immunohistochemical analysis
  • Immunophenotyping
  • Non-Hodgkin's lymphoma
  • ADP ribosyl cyclase/cyclic ADP ribose hydrolase 1
  • anaplastic lymphoma kinase
  • CD2 antigen
  • CD20 antigen
  • CD23 antigen
  • CD3 antigen
  • CD34 antigen
  • CD4 antigen
  • CD5 antigen
  • CD68 antigen
  • CD7 antigen
  • CD79a antigen
  • CD8 antigen
  • common acute lymphoblastic leukemia antigen
  • cyclin D1
  • immunoglobulin kappa chain
  • immunoglobulin lambda chain
  • interferon regulatory factor 4
  • Ki 67 antigen
  • leukosialin
  • protein bcl 2
  • protein bcl 6
  • syndecan 1
  • T lymphocyte receptor alpha chain
  • T lymphocyte receptor beta chain
  • T lymphocyte receptor delta chain
  • T lymphocyte receptor gamma chain
  • transcription factor PAX5
  • tumor necrosis factor receptor superfamily member 8
  • unindexed drug
  • Article
  • bone marrow biopsy
  • bone marrow cancer
  • cancer infiltration
  • controlled study
  • cytofluorometry
  • diagnostic test accuracy study
  • diagnostic value
  • histopathology
  • human
  • human cell
  • human tissue
  • immunohistochemistry
  • immunophenotyping
  • intermethod comparison
  • lymphocytic infiltration
  • major clinical study
  • nonhodgkin lymphoma
  • priority journal
  • retrospective study
  • bone marrow
  • bone marrow examination
  • comparative study
  • flow cytometry
  • pathology
  • procedures
  • tumor invasion
  • Bone Marrow
  • Bone Marrow Examination
  • Flow Cytometry
  • Humans
  • Immunohistochemistry
  • Lymphoma, Non-Hodgkin
  • Neoplasm Invasiveness
  • Retrospective Studies


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