Valutazione citofluorimetrica di cellule dendritiche circolanti in pazienti affetti da sarcoma di Kaposi mediterraneo

Translated title of the contribution: Cytofluorimetric evaluation of peripheral blood dendritic cells in patients with mediterranean Kaposi's sarcoma

M. Vaccari, S. Della Bella, L. Brambilla, S. Ferrucci, S. Nicola, E. Berti, V. Boneschi, M. L. Villa

Research output: Contribution to journalArticle

Abstract

Aim. Kaposi's sarcoma (KS) is a lympho-angioproliferative disorder characterized by angiomatous nodules and plaques that mainly affect the skin. The disease is consistently associated with human herpesvirus-8 (HHV8) and with a state of preexistent immunosupression. Dendritic cells (DCs) have an instrumental role in the activation and function of both innate and adaptative immune responses. At least 2 distinct subsets have been characterized in peripheral blood based on phenotypic markers: myeloid DCs (CD11c+), associated with Ag uptake, T cell activation and ability to secrete IL-12, and plasmacytoid DCs, high virus-induced IFN-alpha producing cells. Because of the role of both DC subtypes in antiviral and antitumor induced responses, we hypothesized that DCs could be involved in the onset and evolution of KS. Methods. Thirty-five patients with mediterranean KS assigned to different clinical stages were compared with 51 healthy control subjects. Peripheral blood DCs were quantified and functionally characterised by flow cytometry directly on whole blood samples. The production of the regulatory cytokines, IL-12 and IL-10, was assessed as intracellular accumulation after incubation with or without lipopolysaccharide (LPS). Results. Myeloid DCs identified as lineage-/HLA-DR+/CD11c+ cells were significantly lower in KS patients than in controls (0.54±0.25 vs 0.69 ±0.26% of the peripheral blood mononuclear cells; p+ DCs were lower in patients with more diffuse disease. Plasmacytoid DCs, identified as lineage-/HLA-DR+/CD123+ cells, were lower in KS patients (0.23±0.19 vs 0.36±0.17; p

Original languageItalian
Pages (from-to)379-386
Number of pages8
JournalMinerva Medica
Volume94
Issue number6
Publication statusPublished - Dec 2003

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Kaposi's Sarcoma
Dendritic Cells
Blood Cells
HLA-DR Antigens
Myeloid Cells
Interleukin-12
Human Herpesvirus 8
Innate Immunity
Interleukin-10
Antiviral Agents
Lipopolysaccharides
Healthy Volunteers
Flow Cytometry
Cytokines
Viruses
T-Lymphocytes
Skin

ASJC Scopus subject areas

  • Medicine(all)

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Valutazione citofluorimetrica di cellule dendritiche circolanti in pazienti affetti da sarcoma di Kaposi mediterraneo. / Vaccari, M.; Della Bella, S.; Brambilla, L.; Ferrucci, S.; Nicola, S.; Berti, E.; Boneschi, V.; Villa, M. L.

In: Minerva Medica, Vol. 94, No. 6, 12.2003, p. 379-386.

Research output: Contribution to journalArticle

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abstract = "Aim. Kaposi's sarcoma (KS) is a lympho-angioproliferative disorder characterized by angiomatous nodules and plaques that mainly affect the skin. The disease is consistently associated with human herpesvirus-8 (HHV8) and with a state of preexistent immunosupression. Dendritic cells (DCs) have an instrumental role in the activation and function of both innate and adaptative immune responses. At least 2 distinct subsets have been characterized in peripheral blood based on phenotypic markers: myeloid DCs (CD11c+), associated with Ag uptake, T cell activation and ability to secrete IL-12, and plasmacytoid DCs, high virus-induced IFN-alpha producing cells. Because of the role of both DC subtypes in antiviral and antitumor induced responses, we hypothesized that DCs could be involved in the onset and evolution of KS. Methods. Thirty-five patients with mediterranean KS assigned to different clinical stages were compared with 51 healthy control subjects. Peripheral blood DCs were quantified and functionally characterised by flow cytometry directly on whole blood samples. The production of the regulatory cytokines, IL-12 and IL-10, was assessed as intracellular accumulation after incubation with or without lipopolysaccharide (LPS). Results. Myeloid DCs identified as lineage-/HLA-DR+/CD11c+ cells were significantly lower in KS patients than in controls (0.54±0.25 vs 0.69 ±0.26{\%} of the peripheral blood mononuclear cells; p+ DCs were lower in patients with more diffuse disease. Plasmacytoid DCs, identified as lineage-/HLA-DR+/CD123+ cells, were lower in KS patients (0.23±0.19 vs 0.36±0.17; p",
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AU - Vaccari, M.

AU - Della Bella, S.

AU - Brambilla, L.

AU - Ferrucci, S.

AU - Nicola, S.

AU - Berti, E.

AU - Boneschi, V.

AU - Villa, M. L.

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AB - Aim. Kaposi's sarcoma (KS) is a lympho-angioproliferative disorder characterized by angiomatous nodules and plaques that mainly affect the skin. The disease is consistently associated with human herpesvirus-8 (HHV8) and with a state of preexistent immunosupression. Dendritic cells (DCs) have an instrumental role in the activation and function of both innate and adaptative immune responses. At least 2 distinct subsets have been characterized in peripheral blood based on phenotypic markers: myeloid DCs (CD11c+), associated with Ag uptake, T cell activation and ability to secrete IL-12, and plasmacytoid DCs, high virus-induced IFN-alpha producing cells. Because of the role of both DC subtypes in antiviral and antitumor induced responses, we hypothesized that DCs could be involved in the onset and evolution of KS. Methods. Thirty-five patients with mediterranean KS assigned to different clinical stages were compared with 51 healthy control subjects. Peripheral blood DCs were quantified and functionally characterised by flow cytometry directly on whole blood samples. The production of the regulatory cytokines, IL-12 and IL-10, was assessed as intracellular accumulation after incubation with or without lipopolysaccharide (LPS). Results. Myeloid DCs identified as lineage-/HLA-DR+/CD11c+ cells were significantly lower in KS patients than in controls (0.54±0.25 vs 0.69 ±0.26% of the peripheral blood mononuclear cells; p+ DCs were lower in patients with more diffuse disease. Plasmacytoid DCs, identified as lineage-/HLA-DR+/CD123+ cells, were lower in KS patients (0.23±0.19 vs 0.36±0.17; p

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KW - Flow cytometry

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