Cytofluorometric assessment of cell cycle progression

Ilio Vitale, Mohamed Jemaà, Lorenzo Galluzzi, Didier Metivier, Maria Castedo, Guido Kroemer

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

One of the most prominent features of cellular senescence, a stress response that prevents the propagation of cells that have accumulated potentially oncogenic alterations, is a permanent loss of proliferative potential. Thus, at odds with quiescent cells, which resume proliferation when stimulated to do so, senescent cells cannot proceed through the cell cycle even in the presence of mitogenic factors. Here, we describe a set of cyto fluorometric techniques for studying how chemical and/or physical stimuli alter the cell cycle in vitro, in both qualitative and quantitative terms. Taken together, these methods allow for the identification of bonafide cytostatic effects as well as for a refined characterization of cell cycle distributions, providing information on proliferation, DNA content as well as on the presence of cell cycle phase-specific markers. At the end of the chapter, a set of guidelines is offered to assist researchers that approach the study of the cell cycle with the interpretation of results.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages93-120
Number of pages28
Volume965
ISBN (Print)9781627032384
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
Volume965
ISSN (Print)10643745

Keywords

  • Cancer
  • Cyclin B1
  • HCT 116
  • Histone H3
  • Mitosis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

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