Cytofluorometric assessment of dendritic cell-mediated uptake of cancer cell apoptotic bodies

Martina Musella, Gwenola Manic, Claudia Galassi, Ilio Vitale, Antonella Sistigu

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Dendritic cells (DCs) are specialized antigen presenting cells (APCs) able to intake and crosspresent antigens (Ags) on major histocompatibility complex (MHC) class I and II molecules to T cells thus initiating primary and memory immune responses. DC-mediated Ag uptake and crosspresentation represent crucial steps toward cancer recognition and eventually elimination. Cytofluorometry is a standardized procedure to study phagocytosis. By fast and reproducible single cell measurements, flow cytometry allows for simultaneous biochemical and functional analyses of Ag intake. In this chapter, we discuss a two-color flow cytometric analysis of DC-mediated uptake of apoptotic bodies. We also show data on the adjuvanticity of Type-I-interferons (Type-I-IFNs) during Ag retention as we offer a guideline and a range of advice on sample preparation and acquisition.

Original languageEnglish
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
DOIs
Publication statusAccepted/In press - Jan 1 2019

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Antigen presenting cells
  • Cytofluorometry
  • Immunogenic cell death
  • Phagocytosis
  • Tumor

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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