Abstract
Dendritic cells (DCs) are specialized antigen presenting cells (APCs) able to intake and crosspresent antigens (Ags) on major histocompatibility complex (MHC) class I and II molecules to T cells thus initiating primary and memory immune responses. DC-mediated Ag uptake and crosspresentation represent crucial steps toward cancer recognition and eventually elimination. Cytofluorometry is a standardized procedure to study phagocytosis. By fast and reproducible single cell measurements, flow cytometry allows for simultaneous biochemical and functional analyses of Ag intake. In this chapter, we discuss a two-color flow cytometric analysis of DC-mediated uptake of apoptotic bodies. We also show data on the adjuvanticity of Type-I-interferons (Type-I-IFNs) during Ag retention as we offer a guideline and a range of advice on sample preparation and acquisition.
| Original language | English |
|---|---|
| Title of host publication | Methods in Enzymology |
| Publisher | Academic Press Inc. |
| DOIs | |
| Publication status | Accepted/In press - Jan 1 2019 |
Publication series
| Name | Methods in Enzymology |
|---|---|
| ISSN (Print) | 0076-6879 |
| ISSN (Electronic) | 1557-7988 |
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Keywords
- Antigen presenting cells
- Cytofluorometry
- Immunogenic cell death
- Phagocytosis
- Tumor
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
Cite this
Cytofluorometric assessment of dendritic cell-mediated uptake of cancer cell apoptotic bodies. / Musella, Martina; Manic, Gwenola; Galassi, Claudia; Vitale, Ilio; Sistigu, Antonella.
Methods in Enzymology. Academic Press Inc., 2019. (Methods in Enzymology).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Cytofluorometric assessment of dendritic cell-mediated uptake of cancer cell apoptotic bodies
AU - Musella, Martina
AU - Manic, Gwenola
AU - Galassi, Claudia
AU - Vitale, Ilio
AU - Sistigu, Antonella
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Dendritic cells (DCs) are specialized antigen presenting cells (APCs) able to intake and crosspresent antigens (Ags) on major histocompatibility complex (MHC) class I and II molecules to T cells thus initiating primary and memory immune responses. DC-mediated Ag uptake and crosspresentation represent crucial steps toward cancer recognition and eventually elimination. Cytofluorometry is a standardized procedure to study phagocytosis. By fast and reproducible single cell measurements, flow cytometry allows for simultaneous biochemical and functional analyses of Ag intake. In this chapter, we discuss a two-color flow cytometric analysis of DC-mediated uptake of apoptotic bodies. We also show data on the adjuvanticity of Type-I-interferons (Type-I-IFNs) during Ag retention as we offer a guideline and a range of advice on sample preparation and acquisition.
AB - Dendritic cells (DCs) are specialized antigen presenting cells (APCs) able to intake and crosspresent antigens (Ags) on major histocompatibility complex (MHC) class I and II molecules to T cells thus initiating primary and memory immune responses. DC-mediated Ag uptake and crosspresentation represent crucial steps toward cancer recognition and eventually elimination. Cytofluorometry is a standardized procedure to study phagocytosis. By fast and reproducible single cell measurements, flow cytometry allows for simultaneous biochemical and functional analyses of Ag intake. In this chapter, we discuss a two-color flow cytometric analysis of DC-mediated uptake of apoptotic bodies. We also show data on the adjuvanticity of Type-I-interferons (Type-I-IFNs) during Ag retention as we offer a guideline and a range of advice on sample preparation and acquisition.
KW - Antigen presenting cells
KW - Cytofluorometry
KW - Immunogenic cell death
KW - Phagocytosis
KW - Tumor
UR - http://www.scopus.com/inward/record.url?scp=85073830084&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85073830084&partnerID=8YFLogxK
U2 - 10.1016/bs.mie.2019.08.005
DO - 10.1016/bs.mie.2019.08.005
M3 - Chapter
AN - SCOPUS:85073830084
T3 - Methods in Enzymology
BT - Methods in Enzymology
PB - Academic Press Inc.
ER -