@inbook{899cee31964b419c8cb9777cb3342523,
title = "Cytofluorometric assessment of dendritic cell-mediated uptake of cancer cell apoptotic bodies",
abstract = "Dendritic cells (DCs) are specialized antigen presenting cells (APCs) able to intake and crosspresent antigens (Ags) on major histocompatibility complex (MHC) class I and II molecules to T cells thus initiating primary and memory immune responses. DC-mediated Ag uptake and crosspresentation represent crucial steps toward cancer recognition and eventually elimination. Cytofluorometry is a standardized procedure to study phagocytosis. By fast and reproducible single cell measurements, flow cytometry allows for simultaneous biochemical and functional analyses of Ag intake. In this chapter, we discuss a two-color flow cytometric analysis of DC-mediated uptake of apoptotic bodies. We also show data on the adjuvanticity of Type-I-interferons (Type-I-IFNs) during Ag retention as we offer a guideline and a range of advice on sample preparation and acquisition.",
keywords = "Antigen presenting cells, Cytofluorometry, Immunogenic cell death, Phagocytosis, Tumor",
author = "Martina Musella and Gwenola Manic and Claudia Galassi and Ilio Vitale and Antonella Sistigu",
year = "2019",
month = jan,
day = "1",
doi = "10.1016/bs.mie.2019.08.005",
language = "English",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
booktitle = "Methods in Enzymology",
address = "United States",
}