Cytofluorometric purification of diploid and tetraploid cancer cells

Maria Castedo, Lorenzo Galluzzi, Ilio Vitale, Laura Senovilla, Didier Métivier, Mohamed Jèmaà, Santiago Rello-Varona, Guido Kroemer

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

During malignant transformation, cells can increase their ploidy and hence become polyploid (mostly tetraploid). Frequently, however, tetraploid cells undergo asymmetric divisions, in turn entailing a reduction in ploidy and the acquisition of a pseudo-diploid, aneuploid state. To investigate such a stepwise aneuploidization process, we developed a cytofluorometric method (based on the heterogeneity in cell size and/or chromatin content) that allows for the cloning and subsequent functional analysis of cells with distinct ploidies. Here, we detail this methodology, which has been instrumental for investigating the functional link between ploidy status and oncogenesis.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages47-63
Number of pages17
Volume761
DOIs
Publication statusPublished - 2011

Publication series

NameMethods in Molecular Biology
Volume761
ISSN (Print)10643745

Keywords

  • Cancer
  • CMFDA
  • CMTMR
  • H2B-GFP
  • p53
  • RKO

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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