TY - CHAP
T1 - Cytofluorometric purification of diploid and tetraploid cancer cells
AU - Castedo, Maria
AU - Galluzzi, Lorenzo
AU - Vitale, Ilio
AU - Senovilla, Laura
AU - Métivier, Didier
AU - Jèmaà, Mohamed
AU - Rello-Varona, Santiago
AU - Kroemer, Guido
PY - 2011
Y1 - 2011
N2 - During malignant transformation, cells can increase their ploidy and hence become polyploid (mostly tetraploid). Frequently, however, tetraploid cells undergo asymmetric divisions, in turn entailing a reduction in ploidy and the acquisition of a pseudo-diploid, aneuploid state. To investigate such a stepwise aneuploidization process, we developed a cytofluorometric method (based on the heterogeneity in cell size and/or chromatin content) that allows for the cloning and subsequent functional analysis of cells with distinct ploidies. Here, we detail this methodology, which has been instrumental for investigating the functional link between ploidy status and oncogenesis.
AB - During malignant transformation, cells can increase their ploidy and hence become polyploid (mostly tetraploid). Frequently, however, tetraploid cells undergo asymmetric divisions, in turn entailing a reduction in ploidy and the acquisition of a pseudo-diploid, aneuploid state. To investigate such a stepwise aneuploidization process, we developed a cytofluorometric method (based on the heterogeneity in cell size and/or chromatin content) that allows for the cloning and subsequent functional analysis of cells with distinct ploidies. Here, we detail this methodology, which has been instrumental for investigating the functional link between ploidy status and oncogenesis.
KW - Cancer
KW - CMFDA
KW - CMTMR
KW - H2B-GFP
KW - p53
KW - RKO
UR - http://www.scopus.com/inward/record.url?scp=79960938506&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79960938506&partnerID=8YFLogxK
U2 - 10.1007/978-1-61779-182-6_3
DO - 10.1007/978-1-61779-182-6_3
M3 - Chapter
C2 - 21755440
AN - SCOPUS:79960938506
SN - 9781617791819
VL - 761
T3 - Methods in Molecular Biology
SP - 47
EP - 63
BT - Methods in Molecular Biology
ER -